Allenmark S, Bergström S, Enerbäck L
Anal Biochem. 1985 Jan;144(1):98-103. doi: 10.1016/0003-2697(85)90089-2.
Based on studies of the reaction between histamine and o-phthalaldehyde in alkaline solution, a method optimized for the determination of histamine in biological samples by means of HPLC and postcolumn o-phthalaldehyde derivatization has been developed. The method permits determination of histamine even at low-picomolar levels. By means of a valve, placed immediately after the column outlet, the eluent stream can be switched between the fluorimetric and an electrochemical detector system whereby electroactive biogenic amines may also be studied under the same chromatographic conditions.
基于对组胺与邻苯二甲醛在碱性溶液中反应的研究，开发了一种通过高效液相色谱法（HPLC）和柱后邻苯二甲醛衍生化来测定生物样品中组胺的优化方法。该方法甚至能够测定低皮摩尔水平的组胺。通过在柱出口后立即设置一个阀，洗脱液流可以在荧光检测器系统和电化学检测器系统之间切换，从而在相同的色谱条件下也可以研究电活性生物胺。
