Gelb J, Weisman Y, Ladman B S, Meir R
Department of Animal and Food Sciences, University of Delaware, Newark, DE 19717, USA.
Avian Pathol. 2005 Jun;34(3):194-203. doi: 10.1080/03079450500096539.
The S1 genes of isolates of avian coronavirus infectious bronchitis virus (IBV) from commercial chickens in the US and Israel (20 isolates from each country) were studied using reverse transcription-polymerase chain reaction restriction fragment length polymorphism and sequencing. Partial sequences spanning the amino terminus region of S1 from amino acid residues 48 to 219, based on the Beaudette strain, were used for analysis. Phylogenetic clustering and high-sequence identity values were used to identify isolates that appeared to be derived from live IBV vaccines used in the two countries. Novel variant strains, unrelated by S1 sequencing and restriction fragment length polymorphism analyses to reference and vaccine strains, were also identified. Based on S1 sequence identity to available vaccines, the potential to use vaccination to control IBV infections was evaluated. Vaccination with commercial live strains Massachusetts (Mass), Arkansas (Ark) or DE/072/92, generally produced immunity against vaccine-related field isolates displaying high S1 sequence similarities (> or = 90%) to the respective vaccine strains. Immunization with a bivalent vaccine containing the Mass and Ark strains provided good cross-protection, averaging 81% against challenge with five variant isolates from the US having amino acid identity values ranging from 62 to 69% to Mass and from 68 to 83% to Ark, respectively. In contrast, the H120 vaccine strain induced low levels of protection, ranging from 25 to 58% against variant field isolates from Israel with amino acid identity values from 65 to 67%.
利用逆转录-聚合酶链反应限制性片段长度多态性分析和测序技术，对来自美国和以色列商业鸡群的禽冠状病毒传染性支气管炎病毒（IBV）分离株（每个国家20株）的S1基因进行了研究。基于Beaudette毒株，分析了S1基因从氨基酸残基48至219的氨基末端区域的部分序列。通过系统发育聚类和高序列同一性值来鉴定似乎源自两国所使用的活IBV疫苗的分离株。还鉴定出了新型变异株，这些变异株通过S1测序和限制性片段长度多态性分析与参考株和疫苗株无关。基于与现有疫苗的S1序列同一性，评估了使用疫苗接种来控制IBV感染的潜力。用商业活毒株马萨诸塞州（Mass）株、阿肯色州（Ark）株或DE/072/92株进行疫苗接种，通常可产生针对与相应疫苗株显示出高S1序列相似性（≥90%）的疫苗相关田间分离株的免疫力。用含有Mass株和Ark株的二价疫苗进行免疫接种可提供良好的交叉保护，平均对来自美国的5株变异分离株的攻击有81%的保护作用，这些分离株与Mass株的氨基酸同一性值为62%至69%，与Ark株的氨基酸同一性值为68%至83%。相比之下，H120疫苗株诱导的保护水平较低，对来自以色列的变异田间分离株的保护率为25%至58%，这些分离株与H120疫苗株的氨基酸同一性值为65%至67%。
