Wood M K, Ladman B S, Preskenis L A, Pope C R, Bautista D, Gelb J
Department of Animal and Food Sciences, Avian Biosciences Center, University of Delaware, Newark, DE 19716-2150, USA.
Avian Dis. 2009 Mar;53(1):119-23. doi: 10.1637/8454-082108-ResNote.1.
Four infectious bronchitis virus (IBV) isolates were recovered from commercial broiler chicken flocks located on the Delmarva Peninsula (east coast of the United States) in the spring of 2006. Sequence analysis of the S1 subunit of the spike glycoprotein gene showed the four isolates were highly related to each other (> or = 99.6% nucleotide identity; > or = 98.9% amino acid identity). Basic local alignment search tool analysis indicated the highest S1 amino acid identity of isolate DMV/5642/06, typical of the four Delmarva (DMV) isolates, was to CA/1737/04, an isolate obtained from broilers in California in 2004. A pathogenicity study conducted, using two-week-old commercial broilers, showed that DMV/5642/06 caused respiratory but not renal (kidney) disease. A vaccination-challenge study in three-week-old specific-pathogen-free leghorn chickens demonstrated that a commercial live attenuated IBV vaccine containing the Massachusetts strain conferred protection against challenge with DMV/5642/06 based on virus reisolation attempts and microscopic pathology.
2006年春季，从位于美国东海岸德尔马瓦半岛的商业肉鸡群中分离出4株传染性支气管炎病毒（IBV）。对刺突糖蛋白基因S1亚基的序列分析表明，这4株分离株彼此高度相关（核苷酸同一性≥99.6%；氨基酸同一性≥98.9%）。基本局部比对搜索工具分析表明，4株德尔马瓦（DMV）分离株中的典型株DMV/5642/06的S1氨基酸同一性最高的是2004年从加利福尼亚州肉鸡中获得的分离株CA/1737/04。使用2周龄商业肉鸡进行的致病性研究表明，DMV/5642/06引起呼吸道疾病，但不引起肾脏疾病。在3周龄无特定病原体的来航鸡中进行的疫苗接种-攻毒研究表明，基于病毒重新分离尝试和显微镜病理学，一种含有马萨诸塞州毒株的商业活减毒IBV疫苗可提供针对DMV/5642/06攻毒的保护。
