Wu Sufang, Chen Gang, Wang Wei, Xu Qian, Gu Hainian, Lu Yunping, Zhou Liping, Du Juan, Li Fujun, Liao Guoning, Ma Ding
Department of Gynecology and Obstetrics, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2005;25(4):451-3, 467. doi: 10.1007/BF02828221.
To investigate the reliability and feasibility of human papillomavirus (HPV) DNA test in cervical scraping smears with polymerase chain reaction (PCR), 131 cases of cervical scraping specimens were collected, and the positive rates and accuracy of HPV infection were determined in normal subjects and cervical cancer patients. GP5+/GP6+ and E7 primer pairs designed for detecting HPV L1 and HPV type 16 E7 were tested in this study. Our results showed that positive rates of HPV DNA in normal population and cervical cancer patients were 32.99% and 73.53% respectively and there was significant difference between them (P < 0.001). In normal subjects, detection rates of HPV DNA with GP5+/GP6+ and E7 primer pairs were 27.84% and 16.49% respectively, with statistically significant difference between them (P > 0.05). However the detection rates in cervical cancer patients were 38.24% and 67.65% for the two markers, with a significant difference found between them (P < 0.05). It is concluded that HPV DNA test with PCR for cervical scraping smears was feasible. GP5+/GP6+ primer pairs may be a useful probe to screen HPV infection in normal population, but they are not sensitive enough in cervical cancer patients. It is suggested that high risk type HPV DNA test was very useful in population with high risk of cervical cancer.
为探讨聚合酶链反应（PCR）检测宫颈刮片中人乳头瘤病毒（HPV）DNA的可靠性和可行性，收集了131例宫颈刮片标本，测定了正常人和宫颈癌患者中HPV感染的阳性率及准确性。本研究检测了用于检测HPV L1的GP5+/GP6+引物对和HPV 16型E7引物对。结果显示，正常人群和宫颈癌患者中HPV DNA的阳性率分别为32.99%和73.53%，两者之间存在显著差异（P < 0.001）。在正常受试者中，GP5+/GP6+引物对和E7引物对检测HPV DNA的阳性率分别为27.84%和16.49%，两者之间差异有统计学意义（P > 0.05）。然而，在宫颈癌患者中，这两种标记物的检测率分别为38.24%和67.65%，两者之间差异有统计学意义（P < 0.05）。结论是，PCR检测宫颈刮片中HPV DNA是可行的。GP5+/GP6+引物对可能是筛查正常人群HPV感染的有用探针，但在宫颈癌患者中敏感性不够。建议高危型HPV DNA检测在宫颈癌高危人群中非常有用。
