Qu W, Jiang G, Cruz Y, Chang C J, Ho G Y, Klein R S, Burk R D
Department of Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
J Clin Microbiol. 1997 Jun;35(6):1304-10. doi: 10.1128/jcm.35.6.1304-1310.1997.
Human papillomavirus (HPV) is an etiologic agent of cervical cancer and is the most common sexually transmitted disease in women. PCR amplification of HPV genomes is the most sensitive method for the detection of cervicovaginal HPV. We have compared the two most commonly used PCR primer sets, MY09/MY11 (MY-PCR) and GP5+/GP6+ (GP+-PCR), for the detection of HPV DNA in cervicovaginal lavage samples from 208 women. Oligonucleotide probes for 39 different HPV types were used. Both primer sets amplified a wide spectrum of HPV genotypes and detected similar overall prevalences of 45% (94 of 208) and 43% (89 of 208), respectively. The MY-PCR system detected 27 of 30 (90%) samples with multiple HPV types, whereas the GP+-PCR system detected 14 of 30 (47%) samples with multiple HPV types. Differences in the detection of HPV types 35, 53, and 61 were noted between the two primer systems. Serial dilution of plasmid templates indicated a 3-log decrease in the amplification of HPV type 35 by MY-PCR and HPV types 53 and 61 by GP+-PCR. These results indicate that although the MY-PCR and GP+-PCR identified nearly equivalent prevalences of HPV in a set of clinical samples, differences in the detection of specific types and infections with multiple types were found. Differences in the sensitivities and characteristics of the PCR systems for the detection of HPV within clinical samples should be considered when comparing data between studies and/or in designing new studies or clinical trials.
人乳头瘤病毒（HPV）是宫颈癌的病原体，也是女性中最常见的性传播疾病。HPV基因组的PCR扩增是检测宫颈阴道HPV最敏感的方法。我们比较了两种最常用的PCR引物组，即MY09/MY11（MY-PCR）和GP5+/GP6+（GP+-PCR），用于检测208名女性宫颈阴道灌洗样本中的HPV DNA。使用了针对39种不同HPV类型的寡核苷酸探针。两种引物组均扩增了广泛的HPV基因型，总体检出率分别为45%（208例中的94例）和43%（208例中的89例），二者相似。MY-PCR系统检测出30例（90%）多种HPV类型样本中的27例，而GP+-PCR系统检测出30例（47%）多种HPV类型样本中的14例。两种引物系统在检测HPV 35、53和61型时存在差异。质粒模板的系列稀释表明，MY-PCR对HPV 35型的扩增以及GP+-PCR对HPV 53和61型的扩增下降了3个对数级。这些结果表明，尽管MY-PCR和GP+-PCR在一组临床样本中鉴定出的HPV总体检出率几乎相同，但在特定类型的检测以及多种类型感染的检测方面存在差异。在比较不同研究的数据和/或设计新的研究或临床试验时，应考虑临床样本中检测HPV的PCR系统在敏感性和特性方面的差异。
