Crystallization and preliminary X-ray study of minor glucoamylase from Aspergillus awamori variant X-100/D27.
作者信息
Golubev A M, Neustroev K N, Aleshin A E, Firsov L M
机构信息
Leningrad Nuclear Physics Institute, U.S.S.R. Academy of Sciences, Gatchina.
出版信息
J Mol Biol. 1992 Jul 5;226(1):271-2. doi: 10.1016/0022-2836(92)90139-b.
PMID:1619656
Abstract
Crystals of the reduced form of glucoamylase were obtained from polyethylene glycol 6000 solution by the hanging-drop method. The protein was treated with alpha-mannosidase to partly remove the sugar component. The crystals belong to the space group P2(1)2(1)2(1) with cell dimensions a = 116.7 A, b = 104.3 A, c = 48.5 A and diffract beyond 2.5 A resolution.
摘要
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