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Crystallization and preliminary X-ray study of minor glucoamylase from Aspergillus awamori variant X-100/D27.

作者信息

Golubev A M, Neustroev K N, Aleshin A E, Firsov L M

机构信息

Leningrad Nuclear Physics Institute, U.S.S.R. Academy of Sciences, Gatchina.

出版信息

J Mol Biol. 1992 Jul 5;226(1):271-2. doi: 10.1016/0022-2836(92)90139-b.

DOI:10.1016/0022-2836(92)90139-b
PMID:1619656
Abstract

Crystals of the reduced form of glucoamylase were obtained from polyethylene glycol 6000 solution by the hanging-drop method. The protein was treated with alpha-mannosidase to partly remove the sugar component. The crystals belong to the space group P2(1)2(1)2(1) with cell dimensions a = 116.7 A, b = 104.3 A, c = 48.5 A and diffract beyond 2.5 A resolution.

摘要

相似文献

1
Crystallization and preliminary X-ray study of minor glucoamylase from Aspergillus awamori variant X-100/D27.
J Mol Biol. 1992 Jul 5;226(1):271-2. doi: 10.1016/0022-2836(92)90139-b.
2
Effect of modification of carbohydrate component on properties of glucoamylase.
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3
Adsorption of glucoamylase from Asp. awamori X-100/D27 on cell walls.泡盛曲霉X-100/D27的糖化酶在细胞壁上的吸附作用。
Biochem Mol Biol Int. 1993 May;30(1):115-20.
4
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FEBS Lett. 1995 Jan 16;358(1):57-61. doi: 10.1016/0014-5793(94)01354-4.
5
Role of the carbohydrate moiety of a glucoamylase from Aspergillus awamori var. kawachi in the digestion of raw starch.泡盛曲霉变种的糖化酶碳水化合物部分在生淀粉消化中的作用。
Biosci Biotechnol Biochem. 1995 Jan;59(1):16-20. doi: 10.1271/bbb.59.16.
6
Refined structure for the complex of 1-deoxynojirimycin with glucoamylase from Aspergillus awamori var. X100 to 2.4-A resolution.泡盛曲霉变种X100来源的葡糖淀粉酶与1-脱氧野尻霉素复合物的精细结构,分辨率达2.4埃。
Biochemistry. 1993 Feb 16;32(6):1618-26. doi: 10.1021/bi00057a028.
7
Microheterogeneity in O-type sugar chains of carbohydrases secreted by Asp. awamori.
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8
Crystals of beta-xylanase from Aspergillus oryzae.
J Mol Biol. 1993 Mar 20;230(2):661-3. doi: 10.1006/jmbi.1993.1177.
9
Refined crystal structures of glucoamylase from Aspergillus awamori var. X100.泡盛曲霉变种X100葡萄糖淀粉酶的精细晶体结构
J Mol Biol. 1994 May 13;238(4):575-91. doi: 10.1006/jmbi.1994.1316.
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Crystallization and preliminary X-ray diffraction studies on recombinant isopenicillin N synthase from Aspergillus nidulans.构巢曲霉重组异青霉素N合酶的结晶及初步X射线衍射研究
Protein Sci. 1995 May;4(5):1007-9. doi: 10.1002/pro.5560040521.

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