Joo Hung-Soo, Hirai Mitsuyo, Shoda Makoto
Chemical Resources Laboratory, Tokyo Institute of Technology, R1-29-4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan.
J Biosci Bioeng. 2005 Aug;100(2):184-91. doi: 10.1263/jbb.100.184.
Alcaligenes faecalis no. 4 has heterotrophic nitrification and aerobic denitrification abilities. By taking the nitrogen balance under different culture conditions, 40-50% of removed NH4+-N was denitrified and about one-half of removed NH4+-N was converted to intracellular nitrogen. The maximum ammonium removal rate of no. 4 (28.9 mg-N/l/h) and its denitrification rate at high-strength NH4+-N of about 1200 ppm in aerated batch experiments at a C/N ratio of 10 were 5-40 times higher than those of other bacteria with the same ability. Only a few percent of the removed ammonium was converted to nitrite, and the main denitrification process was speculated to be via hydroxylamine which was produced by ammonium oxidation.
粪产碱菌4号具有异养硝化和好氧反硝化能力。通过测定不同培养条件下的氮平衡，去除的NH4+-N中有40-50%通过反硝化作用去除，约一半的去除NH4+-N转化为细胞内氮。在C/N比为10的曝气间歇实验中，4号菌株的最大铵去除率（28.9 mg-N/l/h）及其在约1200 ppm高强度NH4+-N下的反硝化率比其他具有相同能力的细菌高5-40倍。只有百分之几的去除铵转化为亚硝酸盐，推测主要反硝化过程是通过铵氧化产生的羟胺进行的。
