College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Bioresour Technol. 2011 Jan;102(2):854-62. doi: 10.1016/j.biortech.2010.09.007. Epub 2010 Sep 7.
Bacterium A1, isolated to enhance nitrogen removal from ammonium-rich wastewater in situ, exhibited an amazing ability to convert ammonium to gaseous nitrogen compounds under fully aerobic conditions, while growing autotrophically or heterotrophically. A1 was identified as Bacillus subtilis by morphological and physiological characteristics, and phylogenetic analysis of its 16S rDNA gene sequence. Nitrogen removal by A1 was analyzed in relation to the ammonium concentration, presence of organic carbon, carbon source, and carbon-to-nitrogen ratio (C/N). The nitrogen balance during 120 h of autotrophic growth in the presence of 104.12±1.27 mg/L NH4+N showed that 20.4±2.7% of NH4+N was removed as gaseous nitrogen compounds, and A1 removed 58.4±4.3% of NH4+N within 60 h of growth in acetate medium at a C/N of 6. A mean ammonium removal rate of 3.52 mg NH4+N/(L h) was achieved in an open wastewater system, indicating great potential of A1 for future full-scale applications.
从富含铵的废水中原位强化脱氮而分离出的细菌 A1，在完全需氧条件下，无论是自养生长还是异养生长，都表现出将铵转化为气态氮化合物的惊人能力。通过形态学和生理学特征以及 16S rDNA 基因序列的系统发育分析，将 A1 鉴定为枯草芽孢杆菌。分析了 A1 与铵浓度、有机碳存在、碳源和碳氮比（C/N）的关系。在存在 104.12±1.27 mg/L NH4+-N 的条件下进行 120 h 自养生长期间的氮平衡表明，20.4±2.7%的 NH4+-N 被转化为气态氮化合物，而 A1 在乙酸盐培养基中以 C/N 为 6 生长 60 h 内去除了 58.4±4.3%的 NH4+-N。在开放式废水系统中实现了平均 3.52 mg NH4+-N/（L h）的铵去除速率，表明 A1 在未来的大规模应用中具有巨大潜力。
