Yu Yamei, Li Lanfen, Zheng Xiaofeng, Liang Yu-He, Su Xiao-Dong
The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, PR China.
Biochim Biophys Acta. 2006 Jan;1764(1):153-6. doi: 10.1016/j.bbapap.2005.08.012. Epub 2005 Sep 12.
Imidazolonepropionase (EC 3.5.2.7) is the third enzyme of the histidine degradation pathway that has been conserved from bacteria to eukaryotes. The enzyme is the only one with unknown three-dimensional structure in this pathway. In this work, Bacillus subtilis imidazolonepropionase (HutI) was expressed in E. coli and purified to homogeneity. After thrombin digestion, high quality crystals were obtained by hanging-drop vapor diffusion method. The best crystal diffracted to 2.0 A and belonged to the space group P2(1) with unit-cell parameters a = 57.73 A, b = 106.34 A, c = 66.47 A, beta = 89.93 degrees .
咪唑啉酮丙酸酶（EC 3.5.2.7）是组氨酸降解途径中的第三种酶，该途径从细菌到真核生物都保守存在。该酶是此途径中唯一三维结构未知的酶。在这项工作中，枯草芽孢杆菌咪唑啉酮丙酸酶（HutI）在大肠杆菌中表达并纯化至同质。经凝血酶消化后，通过悬滴气相扩散法获得了高质量晶体。最佳晶体衍射至2.0埃，属于空间群P2(1)，晶胞参数为a = 57.73埃，b = 106.34埃，c = 66.47埃，β = 89.93° 。
