枯草芽孢杆菌蛋白YtlP的制备、结晶及X射线初步分析
Preparation, crystallization and preliminary X-ray analysis of protein YtlP from Bacillus subtilis.
作者信息
Liu Cong, Li Dan, Hederstedt Lars, Li Lanfen, Liang Yu He, Su Xiao Dong
机构信息
National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, People's Republic of China.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt 10):967-9. doi: 10.1107/S174430910603199X. Epub 2006 Sep 19.
Bacillus subtilis YtlP is a protein that is predicted to belong to the bacterial and archael 2'-5' RNA-ligase family. It contains 183 residues and two copies of the HXTX sequence motif conserved among proteins belonging to this family. In order to determine the structure of YtlP and to compare it with the paralogue YjcG and identified 2'-5' RNA ligases, the gene ytlP was amplified from B. subtilis genomic DNA and cloned into expression vector pET-21a. The soluble protein was produced in Escherichia coli, purified to homogeneity and crystals suitable for X-ray analysis were obtained. The crystal diffracted to 2.0 A and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 34.16, b = 48.54, c = 105.75 A.
枯草芽孢杆菌YtlP是一种预计属于细菌和古菌2'-5'RNA连接酶家族的蛋白质。它含有183个残基以及在该家族蛋白质中保守的两个HXTX序列基序拷贝。为了确定YtlP的结构并将其与旁系同源物YjcG以及已鉴定的2'-5'RNA连接酶进行比较，从枯草芽孢杆菌基因组DNA中扩增出ytlP基因，并将其克隆到表达载体pET-21a中。在大肠杆菌中产生了可溶性蛋白质，将其纯化至同质，并获得了适合X射线分析的晶体。该晶体的衍射分辨率为2.0 Å，属于空间群P2(1)2(1)2(1)，晶胞参数为a = 34.16，b = 48.54，c = 105.75 Å。
Zotero 插件