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1
Cnu, a novel oriC-binding protein of Escherichia coli.Cnu，一种新型的大肠杆菌oriC结合蛋白。
J Bacteriol. 2005 Oct;187(20):6998-7008. doi: 10.1128/JB.187.20.6998-7008.2005.
2
Structure of the nucleoid-associated protein Cnu reveals common binding sites for H-NS in Cnu and Hha.类核相关蛋白Cnu的结构揭示了Cnu和Hha中H-NS的共同结合位点。
Biochemistry. 2008 Feb 19;47(7):1993-2001. doi: 10.1021/bi701914t. Epub 2008 Jan 12.
3
YdgT, the Hha paralogue in Escherichia coli, forms heteromeric complexes with H-NS and StpA.YdgT是大肠杆菌中与Hha同源的蛋白，它与H-NS和StpA形成异源复合物。
Mol Microbiol. 2004 Oct;54(1):251-63. doi: 10.1111/j.1365-2958.2004.04268.x.
4
N9L and L9N mutations toggle Hha binding and hemolysin regulation by Escherichia coli and Vibrio cholerae H-NS.N9L和L9N突变可通过大肠杆菌和霍乱弧菌的H-NS来切换Hha的结合以及溶血素调节。
FEBS Lett. 2009 Sep 3;583(17):2911-6. doi: 10.1016/j.febslet.2009.07.054. Epub 2009 Aug 4.
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Functional replacement of the oligomerization domain of H-NS by the Hha protein of Escherichia coli.大肠杆菌的Hha蛋白对H-NS寡聚结构域的功能替代。
J Bacteriol. 2005 Aug;187(15):5452-9. doi: 10.1128/JB.187.15.5452-5459.2005.
6
A mutational study of Cnu reveals attractive forces between Cnu and H-NS.Cnu 突变研究揭示了 Cnu 和 H-NS 之间的吸引力。
Mol Cells. 2012 Feb;33(2):211-6. doi: 10.1007/s10059-012-0006-5. Epub 2012 Feb 15.
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A single residue mutation in Hha preserving structure and binding to H-NS results in loss of H-NS mediated gene repression properties.Hha中一个保留结构并与H-NS结合的单残基突变导致H-NS介导的基因抑制特性丧失。
FEBS Lett. 2008 Sep 3;582(20):3139-44. doi: 10.1016/j.febslet.2008.07.037. Epub 2008 Aug 18.
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The Escherichia coli SOS gene sbmC is regulated by H-NS and RpoS during the SOS induction and stationary growth phase.大肠杆菌SOS基因sbmC在SOS诱导和稳定生长期受H-NS和RpoS调控。
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New non-detrimental DNA-binding mutants of the Escherichia coli initiator protein DnaA.大肠杆菌起始蛋白DnaA的新型非有害DNA结合突变体
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Evidence for direct protein-protein interaction between members of the enterobacterial Hha/YmoA and H-NS families of proteins.肠道细菌Hha/YmoA蛋白家族与H-NS蛋白家族成员之间直接蛋白质-蛋白质相互作用的证据。
J Bacteriol. 2002 Feb;184(3):629-35. doi: 10.1128/JB.184.3.629-635.2002.

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YmoA functions as a molecular stress sensor in Yersinia.YmoA在耶尔森氏菌中作为分子应激传感器发挥作用。
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Evolutionary and functional divergence of Sfx, a plasmid-encoded H-NS homolog, underlies the regulation of IncX plasmid conjugation.质粒编码的H-NS同源物Sfx的进化和功能差异是IncX质粒接合调控的基础。
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Escherichia coli cell factories with altered chromosomal replication scenarios exhibit accelerated growth and rapid biomass production.具有改变的染色体复制场景的大肠杆菌细胞工厂表现出加速的生长和快速的生物量生产。
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Is Different from Other Members of : Monoploidy, Overlapping Replication Cycle, and Unique Segregation Pattern.与其他成员不同：单倍体、重叠复制周期和独特的分离模式。
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Inactivation of Individual SeqA Binding Sites of the E. coli Origin Reveals Robustness of Replication Initiation Synchrony.大肠杆菌染色体复制起点处单个SeqA结合位点的失活揭示了复制起始同步性的稳健性。
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Multiple DNA Binding Proteins Contribute to Timing of Chromosome Replication in E. coli.多种 DNA 结合蛋白有助于大肠杆菌中染色体的复制定时。
Front Mol Biosci. 2016 Jun 28;3:29. doi: 10.3389/fmolb.2016.00029. eCollection 2016.
8
oriC-encoded instructions for the initiation of bacterial chromosome replication.oriC编码的细菌染色体复制起始指令。
Front Microbiol. 2015 Jan 6;5:735. doi: 10.3389/fmicb.2014.00735. eCollection 2014.
9
The CnuK9E H-NS complex antagonizes DNA binding of DicA and leads to temperature-dependent filamentous growth in E. coli.CnuK9E H-NS 复合物拮抗 DicA 的 DNA 结合，并导致大肠杆菌中温度依赖性的丝状生长。
PLoS One. 2012;7(9):e45236. doi: 10.1371/journal.pone.0045236. Epub 2012 Sep 13.
10
A mutational study of Cnu reveals attractive forces between Cnu and H-NS.Cnu 突变研究揭示了 Cnu 和 H-NS 之间的吸引力。
Mol Cells. 2012 Feb;33(2):211-6. doi: 10.1007/s10059-012-0006-5. Epub 2012 Feb 15.

本文引用的文献

1
YdgT, the Hha paralogue in Escherichia coli, forms heteromeric complexes with H-NS and StpA.YdgT是大肠杆菌中与Hha同源的蛋白，它与H-NS和StpA形成异源复合物。
Mol Microbiol. 2004 Oct;54(1):251-63. doi: 10.1111/j.1365-2958.2004.04268.x.
2
Escherichia coli prereplication complex assembly is regulated by dynamic interplay among Fis, IHF and DnaA.大肠杆菌复制前复合体的组装受Fis、IHF和DnaA之间动态相互作用的调控。
Mol Microbiol. 2004 Mar;51(5):1347-59. doi: 10.1046/j.1365-2958.2003.03906.x.
3
Two discriminatory binding sites in the Escherichia coli replication origin are required for DNA strand opening by initiator DnaA-ATP.大肠杆菌复制起点中的两个识别性结合位点是引发剂DnaA-ATP打开DNA链所必需的。
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2811-6. doi: 10.1073/pnas.0400340101. Epub 2004 Feb 20.
4
DpiA binding to the replication origin of Escherichia coli plasmids and chromosomes destabilizes plasmid inheritance and induces the bacterial SOS response.DpiA与大肠杆菌质粒和染色体的复制起点结合会破坏质粒遗传的稳定性，并诱导细菌的SOS反应。
J Bacteriol. 2003 Oct;185(20):6025-31. doi: 10.1128/JB.185.20.6025-6031.2003.
5
Temperature- and H-NS-dependent regulation of a plasmid-encoded virulence operon expressing Escherichia coli hemolysin.温度和H-NS依赖性调控表达大肠杆菌溶血素的质粒编码毒力操纵子。
J Bacteriol. 2002 Sep;184(18):5058-66. doi: 10.1128/JB.184.18.5058-5066.2002.
6
Effect of different concentrations of H-NS protein on chromosome replication and the cell cycle in Escherichia coli.不同浓度的H-NS蛋白对大肠杆菌染色体复制及细胞周期的影响
J Bacteriol. 2002 Apr;184(7):1843-50. doi: 10.1128/JB.184.7.1843-1850.2002.
7
Evidence for direct protein-protein interaction between members of the enterobacterial Hha/YmoA and H-NS families of proteins.肠道细菌Hha/YmoA蛋白家族与H-NS蛋白家族成员之间直接蛋白质-蛋白质相互作用的证据。
J Bacteriol. 2002 Feb;184(3):629-35. doi: 10.1128/JB.184.3.629-635.2002.
8
CspD, a novel DNA replication inhibitor induced during the stationary phase in Escherichia coli.CspD，一种在大肠杆菌稳定期诱导产生的新型DNA复制抑制剂。
Mol Microbiol. 2001 Mar;39(6):1572-84. doi: 10.1046/j.1365-2958.2001.02345.x.
9
Mechanism of origin unwinding: sequential binding of DnaA to double- and single-stranded DNA.复制起点解旋机制：DnaA蛋白与双链及单链DNA的顺序结合
EMBO J. 2001 Mar 15;20(6):1469-76. doi: 10.1093/emboj/20.6.1469.
10
The arc two-component signal transduction system inhibits in vitro Escherichia coli chromosomal initiation.弧双组分信号转导系统抑制体外大肠杆菌染色体起始。
J Biol Chem. 2001 Mar 30;276(13):9917-23. doi: 10.1074/jbc.M008629200. Epub 2000 Dec 22.

Cnu，一种新型的大肠杆菌oriC结合蛋白。

Cnu, a novel oriC-binding protein of Escherichia coli.

作者信息

Kim Myung Suk, Bae Sung-Hun, Yun Sang Hoon, Lee Hee Jung, Ji Sang Chun, Lee Ji Hyun, Srivastava Preeti, Lee Seol-Hoon, Chae Huiseok, Lee Younghoon, Choi Byong-Seok, Chattoraj Dhruba K, Lim Heon M

机构信息

Department of Biology, School of Biological Sciences and Biotechnology, Chungnam National University, Taejon, 305-764 Korea.

出版信息

J Bacteriol. 2005 Oct;187(20):6998-7008. doi: 10.1128/JB.187.20.6998-7008.2005.

DOI:10.1128/JB.187.20.6998-7008.2005
PMID:16199570
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1251610/
Abstract

We have found, using a newly developed genetic method, a protein (named Cnu, for oriC-binding nucleoid-associated) that binds to a specific 26-base-pair sequence (named cnb) in the origin of replication of Escherichia coli, oriC. Cnu is composed of 71 amino acids (8.4 kDa) and shows extensive amino acid identity to a group of proteins belonging to the Hha/YmoA family. Cnu was previously discovered as a protein that, like Hha, complexes with H-NS in vitro. Our in vivo and in vitro assays confirm the results and further suggest that the complex formation with H-NS is involved in Cnu/Hha binding to cnb. Unlike the hns mutants, elimination of either the cnu or hha gene did not disturb the growth rate, origin content, and synchrony of DNA replication initiation of the mutants compared to the wild-type cells. However, the cnu hha double mutant was moderately reduced in origin content. The Cnu/Hha complex with H-NS thus could play a role in optimal activity of oriC.

摘要

我们使用一种新开发的遗传学方法发现了一种蛋白质（命名为Cnu，即与oriC结合的类核相关蛋白），它能与大肠杆菌复制起点oriC中一段特定的26个碱基对的序列（命名为cnb）结合。Cnu由71个氨基酸组成（8.4 kDa），与属于Hha/YmoA家族的一组蛋白质具有广泛的氨基酸同源性。Cnu此前被发现是一种在体外与H-NS形成复合物的蛋白质，就像Hha一样。我们的体内和体外实验证实了这些结果，并进一步表明与H-NS形成复合物参与了Cnu/Hha与cnb的结合。与hns突变体不同，与野生型细胞相比，敲除cnu或hha基因并不会影响突变体的生长速率、复制起点含量以及DNA复制起始的同步性。然而，cnu hha双突变体的复制起点含量略有降低。因此，与H-NS形成的Cnu/Hha复合物可能在oriC的最佳活性中发挥作用。