Petit Pauline C M, Pible Olivier, Eesbeeck Valérie Van, Alban Claude, Steinmetz Gérard, Mysara Mohamed, Monsieurs Pieter, Armengaud Jean, Rivasseau Corinne
Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), CNRS, INRAE, Université Grenoble Alpes, F-38054 Grenoble, France.
Département Médicaments et Technologies pour la Santé (DMTS), CEA, INRAE, SPI, Université Paris-Saclay, F-30200 Bagnols-sur-Cèze, France.
Microorganisms. 2020 Nov 25;8(12):1857. doi: 10.3390/microorganisms8121857.
The pools of nuclear reactor facilities constitute harsh environments for life, bathed with ionizing radiation, filled with demineralized water and containing toxic radioactive elements. The very few studies published to date have explored water pools used to store spent nuclear fuels. Due to access restrictions and strong handling constraints related to the high radioactivity level, nothing is presently known about life in water pools that directly cool nuclear cores. In this work, we investigated the microbial communities in the cooling pool of the French Osiris nuclear reactor using direct meta-omics approaches, namely, DNA metabarcoding and proteotyping based on 16S ribosomal RNA gene sequencing and on peptide analysis, respectively. We identified 25 genera in the highly radioactive core water supply during operation with radionuclide activity higher than 3 × 10 Bq/m. The prevailing genera and at operation were supplanted by , , and during shutdown. might use dihydrogen produced by water radiolysis as an energy source.
核反应堆设施的水池对生命而言构成了恶劣环境，浸泡在电离辐射中，充满去离子水且含有有毒放射性元素。迄今为止发表的极少数研究探讨了用于储存乏核燃料的水池。由于进入限制以及与高放射性水平相关的严格操作限制，目前对于直接冷却核芯的水池中的生命情况一无所知。在这项工作中，我们使用直接宏组学方法，即分别基于16S核糖体RNA基因测序的DNA宏条形码分析和基于肽分析的蛋白质组学分析，对法国奥西里斯核反应堆的冷却水池中的微生物群落进行了研究。我们在运行期间放射性核素活性高于3×10贝克勒尔/立方米的高放射性堆芯供水系统中鉴定出了25个属。运行期间占主导地位的属在停堆期间被 、 和 所取代。 可能将水辐射分解产生的氢气用作能源。
