Fa Xian'en, Lixia Wang, Hou Jianfeng, Zhang Ruicheng, Wang Haiyong, Yang Chenyuan
Department of Cardiac' Surgery, Union Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2005;25(3):307-9. doi: 10.1007/BF02828151.
Some biological characteristics of human bone marrow mesenchymal stem cells (MSCs) cultured in vitro were observed. hMSCs were isolated from bone marrow and purified by density gradient centrifugation method, and then cultured in vitro. The proliferation and growth characteristics of hMSCs were observed in primary and passage culture. MSCs of passage 3 were examined for the purify by positive rate of CD29 and CD44 through flow cytometry. Human bone marrow MSCs showed active proliferation capacity in vitro. The purify of MSCs separated by our method was higher than 90%. It was concluded that hMSCs have been successfully cultured and expanded effectively. It provided a foundation for further investigation and application of MSCs.
观察了体外培养的人骨髓间充质干细胞（MSCs）的一些生物学特性。从骨髓中分离出人骨髓间充质干细胞，采用密度梯度离心法进行纯化，然后进行体外培养。在原代培养和传代培养中观察人骨髓间充质干细胞的增殖和生长特性。通过流式细胞术检测第3代MSCs的CD29和CD44阳性率以评估其纯度。人骨髓间充质干细胞在体外显示出活跃的增殖能力。用我们的方法分离得到的MSCs纯度高于90%。结论是人骨髓间充质干细胞已成功培养并有效扩增。这为进一步研究和应用MSCs奠定了基础。
