Zhao Jun, Ding Hai-shu, Ruan Man-qi, Kime R, Chance B
Department of Biomedical Engineering, Tsinghua University, Beijing 100084, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Jun;25(6):861-5.
The frequency-domain near-infrared spectrometry (NIRS) is capable of measuring the absolute absorption and reduced scattering coefficient of tissue noninvasively. This allows the quantitation of tissue hemoglobin concentration which reflects the balance between oxygen delivery and oxygen utilization of the skeletal muscle. Phosphorus magnetic resonance spectroscopy (31P-MRS) has become a gold standard of noninvasive measurement of human skeletal muscle metabolism. The rate of phosphocreatine (PCr) resynthesis during recovery is an indicator of the rate of oxidative metabolism. The purpose of the present study was the effect of lower intracellular pH (pHi) on PCr and oxygenation recovery. The preliminary results of plantar flexion experiment on a healthy male subject show that both PCr resynthesis and reoxygenation were very much prolonged by severe acidosis (pHi = 6.42).
频域近红外光谱法（NIRS）能够无创测量组织的绝对吸收系数和约化散射系数。这使得定量测定反映骨骼肌氧输送与氧利用平衡的组织血红蛋白浓度成为可能。磷磁共振波谱法（31P-MRS）已成为无创测量人体骨骼肌代谢的金标准。恢复过程中磷酸肌酸（PCr）的再合成速率是氧化代谢速率的一个指标。本研究的目的是探讨较低的细胞内pH值（pHi）对PCr和氧合恢复的影响。对一名健康男性受试者进行的跖屈实验的初步结果表明，严重酸中毒（pHi = 6.42）会使PCr再合成和再氧合过程都大大延长。
