[中国患者脑脊液中日本脑炎病毒株全长基因组的分子特征分析]

[Molecular characterization of full-length genome of Japanese encephalitis virus strain patient's cerebrospinal fluid in China].

作者信息

Pan Xiao-Ling, Wang Huan-Yu, Fu Shi-Hong, Hian Na, Liu Hai-Zhou, Rayner Simon, Hang Guo-Dong

机构信息

Institution for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Bejing 100052, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Feb;23(1):26-8.

PMID:19799010

Abstract

OBJECTIVE

To sequence and analyze the whole genome of Japanese encephalitis virus (JEV) strain named 47 which was isolated from patient's cerebrospinal fluid sample in Heilongjiang province in 1950.

METHODS

RNA was extracted from the recovery strain 47 and amplified with self-designed JEV genome sequencing primers. The differentiation analysis for nucleotides and coding amino acids and phylogenetic analysis were performed by the software of DNAStar, Modeltest, and Phylip.

RESULTS

The whole genome of strain 47 has 10,977 nucleotides. An open reading frame from 95 to 10,391 including 10,296 nucleotides is capable of coding a 3432 amino acid polyprotein. The nucleotide difference between strain 47 and 5 vaccine strains is 2.4%-4.4%, the amino acid difference between strain 47 and 5 vaccine strains is 0.3%-1.1%. The best evolution model for the whole genome is GTR + I + G. Based on the phylogenetic analysis, strain 47 belongs to the genotype III JEV.

CONCLUSION

Strain 47 is highly conserved on whole genome nucleotide and amino acid sequence. And it is belongs to the genotype III JEV.

摘要

目的

对1950年从黑龙江省一名患者脑脊液样本中分离出的日本脑炎病毒（JEV）47株进行全基因组测序和分析。

方法

从复苏的47株病毒中提取RNA，并用自行设计的JEV基因组测序引物进行扩增。利用DNAStar、Modeltest和Phylip软件进行核苷酸和编码氨基酸的差异分析以及系统发育分析。

结果

47株病毒的全基因组有10977个核苷酸。一个从95到10391位包含10296个核苷酸的开放阅读框能够编码一个3432个氨基酸的多聚蛋白。47株病毒与5株疫苗株之间的核苷酸差异为2.4%-4.4%，氨基酸差异为0.3%-1.1%。全基因组的最佳进化模型是GTR+I+G。基于系统发育分析，47株病毒属于JEV基因型III。