Guo Lan-Ping, Wang Zhi-Gang, Liang Biao, Wu Bin, Zhou Ai-Lian, Peng Bo
Institute of Respiratory Disease, Guangdong Medical Collage, The Affiliated Hospital of Guangdong Medical College, Zharjiang 524001, China.
Zhongguo Zhong Yao Za Zhi. 2005 Aug;30(15):1188-91.
To observe the effect of Dansen injection on the experimental emphysema in rabbits.
Thirty-six rabbits were randomized into emphysema group (n = 12), Dansen injection treated group (n = 12) and alpha1-antitrypsin(alpha1-AT) treated group (n = 12). The animal model of emphysema was induced by intratracheal instillation of porcine pancreatic elastase. Dansen injection and alpha1-ATwere instilled intratracheal in two treated group after 14 days with porcine pancreatic elastase, respectively, once a week, to continue for four weeks. The level of alpha1-AT in serum and in bronchoalveolar lavage fluid (BALF) were analyzed in different times. The mean linear intercept value (MLIV) and the numbers of alveolar per square (NAPS) of all groups were compared after eight weeks with porcine pancreatic elastase.
The levels of alpha1-AT in BALF were significantly different between treated groups and emphysema group after two weeks treatment, alpha1-AT levels of treated groups were more increased than those of emphysema group (P < 0.01). The levels of alpha1-AT in serum were similar at same times in different groups (P > 0.05), but were great different in different times. The MLIV and the NAPS were significantly different from emphysema group to treated groups in sixth and eighth weeks (P < 0.01), there is no difference between dancen group and alpha1-AT group.
The contents of alpha1-AT in local pulmonary tissue could be improved by Dansen injection through intratracheal instillation during the information of emphysema in rabbits. The effect of Dansen injection and alpha1-AT on preventing formation of emphysema is similar.
观察丹参注射液对兔实验性肺气肿的影响。
36只兔随机分为肺气肿组（n = 12）、丹参注射液治疗组（n = 12）和α1-抗胰蛋白酶（α1-AT）治疗组（n = 12）。通过气管内滴注猪胰弹性蛋白酶诱导肺气肿动物模型。在滴注猪胰弹性蛋白酶14天后，分别对两个治疗组进行气管内滴注丹参注射液和α1-AT，每周1次，持续4周。在不同时间分析血清和支气管肺泡灌洗液（BALF）中α1-AT的水平。在滴注猪胰弹性蛋白酶8周后比较所有组的平均线性截距值（MLIV）和每平方肺泡数（NAPS）。
治疗2周后，治疗组和肺气肿组BALF中α1-AT水平有显著差异，治疗组α1-AT水平比肺气肿组升高更明显（P < 0.01）。不同组血清中α1-AT水平在同一时间相似（P > 0.05），但在不同时间差异很大。在第6周和第8周，肺气肿组与治疗组的MLIV和NAPS有显著差异（P < 0.01），丹参组和α1-AT组之间无差异。
在兔肺气肿形成过程中，通过气管内滴注丹参注射液可提高局部肺组织中α1-AT的含量。丹参注射液和α1-AT预防肺气肿形成的效果相似。
