Huang Qing, Fu Wei-Ling
Department of Laboratory Medicine, Southwest Hospital, the Third Military Medical University, Chongqing, China.
Clin Chem Lab Med. 2005;43(8):841-2. doi: 10.1515/CCLM.2005.141.
Ethidium bromide (EB) is a mutagen and toxin that is widely used in the laboratory for visualization of nucleic acids. Safer nucleic acid stains, such as SYBR Gold, SYBR Green, GoldView, GeneFinder, and GoldStar, have been developed. However, there has been no systematic comparative analysis of the staining efficiencies of these dyes. In the present study, SYBR Gold, SYBR Green I, GoldView and EB were compared. Although both SYBR Gold and SYBR Green alter electrophoretic mobility and thus DNA size estimates, they are cost-effective alternatives to EB. SYBR Gold was more sensitive than SYBR Green I at detecting short fragments, but 50-bp bands were clearly visible using either dye when visualized with a long integration time. SYBR Gold or SYBR Green I are sensitive and relatively safe alternatives to EB. In our laboratory, the SYBR Gold method is now used routinely by all members of our group with great consistency and success.
溴化乙锭（EB）是一种诱变剂和毒素，在实验室中广泛用于核酸可视化。现已开发出更安全的核酸染色剂，如SYBR Gold、SYBR Green、GoldView、GeneFinder和GoldStar。然而，尚未对这些染料的染色效率进行系统的比较分析。在本研究中，对SYBR Gold、SYBR Green I、GoldView和EB进行了比较。尽管SYBR Gold和SYBR Green都会改变电泳迁移率，从而影响DNA大小估计，但它们是EB的经济有效替代品。在检测短片段时，SYBR Gold比SYBR Green I更灵敏，但当长时间曝光时，使用这两种染料都能清晰看到50bp的条带。SYBR Gold或SYBR Green I是EB的灵敏且相对安全的替代品。在我们实验室，现在我们团队的所有成员都常规使用SYBR Gold方法，结果高度一致且很成功。
