一株源自人类胃肠道微生物群的嗜酸乳杆菌通过引发致命的细菌膜损伤，诱导对肠道致病性鼠伤寒沙门氏菌的杀伤作用。
A Lactobacillus acidophilus strain of human gastrointestinal microbiota origin elicits killing of enterovirulent Salmonella enterica Serovar Typhimurium by triggering lethal bacterial membrane damage.
作者信息
Coconnier-Polter Marie-Hélène, Liévin-Le Moal Vanessa, Servin Alain L
机构信息
Faculté de Pharmacie, Unité Inserm 510, F-92296 Chātenay-Malabry, France.
出版信息
Appl Environ Microbiol. 2005 Oct;71(10):6115-20. doi: 10.1128/AEM.71.10.6115-6120.2005.
Abstract
The human gastrointestinal microbiota produces antagonistic activities against gastrointestinal bacterial pathogens. We undertook a study to investigate the mechanism(s) by which a Lactobacillus acidophilus strain of human microbiota origin antagonizes the gram-negative enteroinvasive pathogen Salmonella enterica serovar Typhimurium. We showed that the cell-free culture supernatant of L. acidophilus strain LB (LB-CFCS) induced the following effects in S. enterica SL1344: (i) a decrease in intracellular ATP that paralleled bacterial death, (ii) the release of lipopolysaccharide, (iii) permeabilization of the bacterial membrane, and (iv) an increase in the sensitivity of Salmonella to the lytic action of sodium dodecyl sulfate. Finally, we showed using two mutant strains of Salmonella, PhoP MS7953s and PmrA JKS1170, that the two-component regulatory systems PhoP-PhoQ and PmrA-PmrB that regulate the mechanisms of resistance to antibacterial agents in Salmonella did not influence the anti-Salmonella effect of LB-CFCS.
摘要
人类胃肠道微生物群对胃肠道细菌病原体具有拮抗活性。我们开展了一项研究，以探究源自人类微生物群的嗜酸乳杆菌菌株拮抗革兰氏阴性肠道侵袭性病原体鼠伤寒沙门氏菌的机制。我们发现，嗜酸乳杆菌菌株LB的无细胞培养上清液（LB-CFCS）对肠炎沙门氏菌SL1344产生了以下影响：（i）细胞内ATP减少，与细菌死亡平行；（ii）脂多糖释放；（iii）细菌膜通透性增加；（iv）沙门氏菌对十二烷基硫酸钠的裂解作用敏感性增加。最后，我们使用两种沙门氏菌突变株PhoP MS7953s和PmrA JKS1170表明，调节沙门氏菌对抗菌剂耐药机制的双组分调节系统PhoP-PhoQ和PmrA-PmrB不影响LB-CFCS的抗沙门氏菌作用。
相似文献
1
A Lactobacillus acidophilus strain of human gastrointestinal microbiota origin elicits killing of enterovirulent Salmonella enterica Serovar Typhimurium by triggering lethal bacterial membrane damage.一株源自人类胃肠道微生物群的嗜酸乳杆菌通过引发致命的细菌膜损伤，诱导对肠道致病性鼠伤寒沙门氏菌的杀伤作用。
Appl Environ Microbiol. 2005 Oct;71(10):6115-20. doi: 10.1128/AEM.71.10.6115-6120.2005.
2
The Lactobacillus plantarum strain ACA-DC287 isolated from a Greek cheese demonstrates antagonistic activity in vitro and in vivo against Salmonella enterica serovar Typhimurium.从希腊奶酪中分离出的植物乳杆菌菌株ACA-DC287在体外和体内均表现出对鼠伤寒沙门氏菌的拮抗活性。
J Appl Microbiol. 2007 Sep;103(3):657-65. doi: 10.1111/j.1365-2672.2007.03293.x.
3
Antagonistic activity of Lactobacillus acidophilus LB against intracellular Salmonella enterica serovar Typhimurium infecting human enterocyte-like Caco-2/TC-7 cells.嗜酸乳杆菌LB对感染人肠上皮样Caco-2/TC-7细胞的胞内鼠伤寒沙门氏菌的拮抗活性。
Appl Environ Microbiol. 2000 Mar;66(3):1152-7. doi: 10.1128/AEM.66.3.1152-1157.2000.
4
pH-, Lactic acid-, and non-lactic acid-dependent activities of probiotic Lactobacilli against Salmonella enterica Serovar Typhimurium.益生菌乳酸杆菌对肠炎沙门氏菌鼠伤寒血清型的pH值、乳酸和非乳酸依赖性活性。
Appl Environ Microbiol. 2005 Oct;71(10):6008-13. doi: 10.1128/AEM.71.10.6008-6013.2005.
5
Impairment of swimming motility by antidiarrheic Lactobacillus acidophilus strain LB retards internalization of Salmonella enterica serovar Typhimurium within human enterocyte-like cells.抗腹泻嗜酸乳杆菌 LB 菌株对游泳运动能力的损害会延缓鼠伤寒沙门氏菌血清型 Typhimurium 在内源肠细胞样细胞中的内化。
Antimicrob Agents Chemother. 2011 Oct;55(10):4810-20. doi: 10.1128/AAC.00418-11. Epub 2011 Aug 8.
6
Individual and co-operative roles of lactic acid and hydrogen peroxide in the killing activity of enteric strain Lactobacillus johnsonii NCC933 and vaginal strain Lactobacillus gasseri KS120.1 against enteric, uropathogenic and vaginosis-associated pathogens.肠内株约翰逊乳杆菌 NCC933 和阴道株加氏乳杆菌 KS120.1 乳酸和过氧化氢在杀灭肠道、尿路和阴道病相关病原体的杀伤活性中的个体和协同作用。
FEMS Microbiol Lett. 2010 Mar;304(1):29-38. doi: 10.1111/j.1574-6968.2009.01887.x. Epub 2009 Dec 24.
7
Lactobacillus acidophilus LAP5 able to inhibit the Salmonella choleraesuis invasion to the human Caco-2 epithelial cell.嗜酸乳杆菌LAP5能够抑制猪霍乱沙门氏菌对人Caco-2上皮细胞的侵袭。
Anaerobe. 2008 Nov;14(5):251-5. doi: 10.1016/j.anaerobe.2008.07.003. Epub 2008 Aug 22.
8
The Salmonella membrane protein IgaA modulates the activity of the RcsC-YojN-RcsB and PhoP-PhoQ regulons.鼠伤寒沙门氏菌膜蛋白IgaA可调节RcsC-YojN-RcsB和PhoP-PhoQ调节子的活性。
J Bacteriol. 2004 Nov;186(22):7481-9. doi: 10.1128/JB.186.22.7481-7489.2004.
9
Salmonella enterica serovar Typhimurium response involved in attenuation of pathogen intracellular proliferation.肠炎沙门氏菌鼠伤寒血清型的反应参与了病原体细胞内增殖的减弱。
Infect Immun. 2001 Oct;69(10):6463-74. doi: 10.1128/IAI.69.10.6463-6474.2001.
10
Hydrogen peroxide production by Lactobacillus johnsonii NCC 533 and its role in anti-Salmonella activity.约氏乳杆菌NCC 533产过氧化氢及其在抗沙门氏菌活性中的作用。
FEMS Microbiol Lett. 2008 Jun;283(2):210-5. doi: 10.1111/j.1574-6968.2008.01176.x. Epub 2008 Apr 22.
引用本文的文献
1
Lactobacilli, a Weapon to Counteract Pathogens through the Inhibition of Their Virulence Factors.乳酸菌，通过抑制其毒力因子来对抗病原体的武器。
J Bacteriol. 2022 Nov 15;204(11):e0027222. doi: 10.1128/jb.00272-22. Epub 2022 Oct 26.
2
Arginine- and Polyamine-Induced Lactic Acid Resistance in Neisseria gonorrhoeae.精氨酸和多胺诱导淋病奈瑟菌对乳酸的抗性
PLoS One. 2016 Jan 25;11(1):e0147637. doi: 10.1371/journal.pone.0147637. eCollection 2016.
3
A gastrointestinal anti-infectious biotherapeutic agent: the heat-treated Lactobacillus LB.一种胃肠道抗感染生物治疗剂：经热处理的乳酸杆菌LB。
Therap Adv Gastroenterol. 2016 Jan;9(1):57-75. doi: 10.1177/1756283X15602831.
4
Anti-infective activities of lactobacillus strains in the human intestinal microbiota: from probiotics to gastrointestinal anti-infectious biotherapeutic agents.人类肠道微生物群中乳酸菌菌株的抗感染活性：从益生菌到胃肠道抗感染生物治疗剂。
Clin Microbiol Rev. 2014 Apr;27(2):167-99. doi: 10.1128/CMR.00080-13.
5
Antagonistic activity of Lactobacillus isolates against Salmonella typhi in vitro.体外分离的乳酸杆菌对伤寒沙门氏菌的拮抗活性。
Biomed Res Int. 2013;2013:680605. doi: 10.1155/2013/680605. Epub 2013 Sep 29.
6
Rapid identification of antifungal compounds against Exserohilum rostratum using high throughput drug repurposing screens.采用高通量药物再利用筛选技术快速鉴定针对罗司氏菌的抗真菌化合物。
PLoS One. 2013 Aug 21;8(8):e70506. doi: 10.1371/journal.pone.0070506. eCollection 2013.
7
A high-throughput screening assay for assessing the viability of Cryptococcus neoformans under nutrient starvation conditions.高通量筛选检测评估新型隐球菌在营养饥饿条件下的活力。
Anal Bioanal Chem. 2013 Aug;405(21):6823-9. doi: 10.1007/s00216-013-7134-4. Epub 2013 Jun 30.
8
Impairment of swimming motility by antidiarrheic Lactobacillus acidophilus strain LB retards internalization of Salmonella enterica serovar Typhimurium within human enterocyte-like cells.抗腹泻嗜酸乳杆菌 LB 菌株对游泳运动能力的损害会延缓鼠伤寒沙门氏菌血清型 Typhimurium 在内源肠细胞样细胞中的内化。
Antimicrob Agents Chemother. 2011 Oct;55(10):4810-20. doi: 10.1128/AAC.00418-11. Epub 2011 Aug 8.
9
Salmonella enterica serovar Typhimurium adhesion and cytotoxicity during epithelial cell stress is reduced by Lactobacillus rhamnosus GG.鼠伤寒沙门氏菌血清型 Typhimurium 在肠上皮细胞应激时的黏附和细胞毒性，可被鼠李糖乳杆菌 GG 所降低。
Gut Pathog. 2009 Jul 9;1(1):14. doi: 10.1186/1757-4749-1-14.
10
Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356.嗜酸乳杆菌ATCC 4356表层中的胞壁质水解酶活性
Appl Environ Microbiol. 2008 Dec;74(24):7824-7. doi: 10.1128/AEM.01712-08. Epub 2008 Oct 17.
本文引用的文献
1
pH-, Lactic acid-, and non-lactic acid-dependent activities of probiotic Lactobacilli against Salmonella enterica Serovar Typhimurium.益生菌乳酸杆菌对肠炎沙门氏菌鼠伤寒血清型的pH值、乳酸和非乳酸依赖性活性。
Appl Environ Microbiol. 2005 Oct;71(10):6008-13. doi: 10.1128/AEM.71.10.6008-6013.2005.
2
Penetration and interactions of the antimicrobial peptide, microcin J25, into uncharged phospholipid monolayers.抗菌肽微菌素J25对不带电荷磷脂单分子层的穿透及相互作用
J Colloid Interface Sci. 2005 May 1;285(1):118-24. doi: 10.1016/j.jcis.2004.11.025.
3
Effects of microcin 24-producing Escherichia coli on shedding and multiple-antimicrobial resistance of Salmonella enterica serotype typhimurium in pigs.产微菌素24的大肠杆菌对猪肠炎沙门氏菌鼠伤寒血清型脱落及多重耐药性的影响。
Am J Vet Res. 2004 Dec;65(12):1616-20. doi: 10.2460/ajvr.2004.65.1616.
4
Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens.乳酸杆菌和双歧杆菌对微生物病原体的拮抗活性。
FEMS Microbiol Rev. 2004 Oct;28(4):405-40. doi: 10.1016/j.femsre.2004.01.003.
5
PhoP-regulated Salmonella resistance to the antimicrobial peptides magainin 2 and polymyxin B.PhoP调控的鼠伤寒沙门氏菌对抗菌肽蛙皮素2和多粘菌素B的抗性。
Mol Microbiol. 2004 Jul;53(1):229-41. doi: 10.1111/j.1365-2958.2004.04107.x.
6
Microflora of the gastrointestinal tract: a review.胃肠道微生物群：综述
Methods Mol Biol. 2004;268:491-502. doi: 10.1385/1-59259-766-1:491.
7
Regulation of Salmonella typhimurium virulence gene expression by cationic antimicrobial peptides.阳离子抗菌肽对鼠伤寒沙门氏菌毒力基因表达的调控
Mol Microbiol. 2003 Oct;50(1):219-30. doi: 10.1046/j.1365-2958.2003.03675.x.
8
Cationic antimicrobial peptides activate a two-component regulatory system, PmrA-PmrB, that regulates resistance to polymyxin B and cationic antimicrobial peptides in Pseudomonas aeruginosa.阳离子抗菌肽激活一种双组分调节系统PmrA-PmrB，该系统调节铜绿假单胞菌对多粘菌素B和阳离子抗菌肽的抗性。
Mol Microbiol. 2003 Oct;50(1):205-17. doi: 10.1046/j.1365-2958.2003.03673.x.
9
Defensins: antimicrobial peptides of innate immunity.防御素：天然免疫的抗菌肽。
Nat Rev Immunol. 2003 Sep;3(9):710-20. doi: 10.1038/nri1180.
10
Effect of EDTA on Salmonella enterica serovar Typhimurium involves a component not assignable to lipopolysaccharide release.乙二胺四乙酸对鼠伤寒沙门氏菌的作用涉及一种无法归因于脂多糖释放的成分。
Microbiology (Reading). 2003 Aug;149(Pt 8):2015-2021. doi: 10.1099/mic.0.26312-0.
Zotero 插件