Weston K
Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
Nucleic Acids Res. 1992 Jun 25;20(12):3043-9. doi: 10.1093/nar/20.12.3043.
The chicken c-myb gene and the v-myb oncogene transduced by avian myeloblastosis virus (AMV) encode DNA binding transcription activators. The DNA binding domain of AMV v-Myb displays a number of amino acid changes relative to c-Myb; v-Myb proteins in which one or more of three crucial residues in the DNA binding domain are mutated to resemble the c-Myb sequence display altered transformation phenotypes. In order to establish whether the spectrum of DNA binding sites which AMV v-Myb can recognise is different from that seen by chicken c-Myb, a site selection protocol was used to derive consensus binding sequences for three variant Myb proteins made in vitro, and also using nuclear extract from the v-myb transformed cell line BM2. The results show that the original consensus binding site defined for v-Myb, YAA-CKG, can be extended to YAACKGHH, and that this new consensus holds for both v-Myb and chicken c-Myb.
鸡的c-myb基因以及由禽成髓细胞瘤病毒（AMV）转导的v-myb癌基因编码DNA结合转录激活因子。相对于c-Myb，AMV v-Myb的DNA结合结构域显示出一些氨基酸变化；DNA结合结构域中三个关键残基中的一个或多个发生突变以类似于c-Myb序列的v-Myb蛋白表现出改变的转化表型。为了确定AMV v-Myb能够识别的DNA结合位点谱是否与鸡c-Myb不同，采用位点选择方案来推导体外制备的三种变体Myb蛋白以及使用来自v-myb转化细胞系BM2的核提取物的共有结合序列。结果表明，为v-Myb定义的原始共有结合位点YAA-CKG可以扩展为YAACKGHH，并且这个新的共有序列对v-Myb和鸡c-Myb都适用。
