Bonfill Mercè, Mangas Susana, Cusidó Rosa M, Osuna Lidia, Piñol M Teresa, Palazón Javier
Laboratorio de Fisiología vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028 Barcelona, Spain.
Biomed Chromatogr. 2006 Feb;20(2):151-3. doi: 10.1002/bmc.564.
The identification of the four principal triterpenoid components of Centella asiatica has been achieved by TLC on silica gel plates and mass spectrometry, as a modification of the method described in the European Pharmacopoeia (5th edn). A combination of ethyl acetate and methanol as the mobile phase was found to be successful in separating these compounds from the rest of the main components of the extract. The spots were detected with anisaldehyde solution. The separated compounds were confirmed by MALDI -TOF mass spectrometry.
通过硅胶板薄层色谱法和质谱法，对积雪草的四种主要三萜类成分进行了鉴定，这是对《欧洲药典》（第5版）所述方法的改进。发现以乙酸乙酯和甲醇的混合物作为流动相，能够成功地将这些化合物与提取物的其他主要成分分离。用茴香醛溶液检测斑点。通过基质辅助激光解吸电离飞行时间质谱法对分离出的化合物进行了确认。
