Yu Quan-Lin, Gao Wen-Yuan, Chen Hai-Xia, Duan Hong-Quan
College of Pharmaceutical Sciences and Biotechnology, Tianjin University, Tianjin 300072, China.
Zhongguo Zhong Yao Za Zhi. 2007 Mar;32(6):503-5.
A HPLC-ELSD method was established for the simultaneous quantitative determination of asiaticoside in Centella asiatica extract.
The column was packed with 5 m HIQ C18 stationary phase. The mobile phase consisted of acetonitrile-water, eluted in gradient mode. The temperature of drift tube was 105 degrees C and the nebulizer nitrogen flow rate was 2.9 L min(-1).
The linear ranges of the asiaticoside were 0.35-7.0 microg. The recovery of the asiaticoside in C. asiatica extract was 94.9% (RSD 1.7%).
The method is reliable, simple, precise and could be used for the quality control of C. asiatica extract.
建立一种高效液相色谱 - 蒸发光散射检测法（HPLC - ELSD），用于同时定量测定积雪草提取物中积雪草苷的含量。
采用5μm HIQ C18固定相填充柱。流动相为乙腈 - 水，采用梯度洗脱模式。漂移管温度为105℃，雾化器氮气流量为2.9 L min⁻¹。
积雪草苷的线性范围为0.35 - 7.0μg。积雪草提取物中积雪草苷的回收率为94.9%（相对标准偏差为1.7%）。
该方法可靠、简便、精确，可用于积雪草提取物的质量控制。
