一种改进的高效液相色谱-紫外检测法，用于同时定量积雪草（伞形科）叶片中的三萜糖苷和苷元。
An improved HPLC-UV method for the simultaneous quantification of triterpenic glycosides and aglycones in leaves of Centella asiatica (L.) Urb (APIACEAE).
作者信息
Rafamantanana M H, Rozet E, Raoelison G E, Cheuk K, Ratsimamanga S U, Hubert Ph, Quetin-Leclercq J
机构信息
Institut Malgache de Recherches Appliquées, BP 3833, 101 Antananarivo, Madagascar.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Aug 1;877(23):2396-402. doi: 10.1016/j.jchromb.2009.03.018. Epub 2009 Mar 19.
The simultaneous quantification of madecassoside, asiaticoside, madecassic acid and asiatic acid in Centella asiatica by HPLC-UV is proposed. Asiaticoside was used as reference for the quantification of heterosides and asiatic acid for aglycones. The evaluation of the extraction efficiency of the four molecules led to use Soxhlet extraction for 8 h. The method was validated and was found to be accurate in the concentration range of 1.0-3.0 mg/ml for asiaticoside and 0.5-2.0 mg/ml for asiatic acid with CV <3% for all investigated compounds. LOD and LOQ were, respectively, 0.0113 and 1.0 mg/ml for asiaticoside and 0.0023 and 0.5 mg/ml for asiatic acid. This method was shown to be convenient for routine analysis of samples of C. asiatica.
本文提出了采用高效液相色谱-紫外检测法（HPLC-UV）同时测定积雪草中羟基积雪草苷、积雪草苷、羟基积雪草酸和积雪草酸的含量。以积雪草苷作为测定总苷类成分的对照品，积雪草酸作为测定苷元的对照品。通过对这四种成分提取效率的评估，最终采用索氏提取法提取8小时。该方法经过验证，结果表明，在1.0-3.0mg/ml的浓度范围内，羟基积雪草苷的测定结果准确，在0.5-2.0mg/ml的浓度范围内，积雪草酸的测定结果准确，所有被研究化合物的变异系数（CV）均<3%。羟基积雪草苷的检测限（LOD）和定量限（LOQ）分别为0.0113和1.0mg/ml，积雪草酸的检测限和定量限分别为0.0023和0.5mg/ml。结果表明，该方法便于对积雪草样品进行常规分析。
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