Lemar Katey M, Passa Ourania, Aon Miguel A, Cortassa Sonia, Müller Carsten T, Plummer Sue, O'Rourke Brian, Lloyd David
Microbiology, Cardiff School of Biosciences, Main Building, Cardiff CF10 3TL, Wales, UK.
The Institute of Molecular Cardiobiology, Johns Hopkins Medicine, 720 Rutland Ave, 844 Ross Building, Baltimore, MD 21205-2195, USA.
Microbiology (Reading). 2005 Oct;151(Pt 10):3257-3265. doi: 10.1099/mic.0.28095-0.
Both the growth and respiration of Candida albicans are sensitive to extracts of Allium sativum and investigations into the anticandidal activities are now focussing on the purified constituents to determine the targets of inhibition. Of particular interest is allyl alcohol (AA), a metabolic product that accumulates after trituration of garlic cloves. Putative targets for AA were investigated by monitoring changes in intracellular responses after exposure of C. albicans cells to AA or a commercially available garlic extract. Two-photon laser scanning microscopy and other techniques were used. Changes typical of oxidative stress--NADH oxidation and glutathione depletion, and increased reactive oxygen species--were observed microscopically and by flow cytometry. Known targets for AA are alcohol dehydrogenases Adh1 and 2 (in the cytosol) and Adh3 (mitochondrial), although the significant decrease in NAD(P)H after addition of AA is indicative of another mechanism of action.
白色念珠菌的生长和呼吸对大蒜提取物敏感，目前针对抗念珠菌活性的研究集中在纯化成分上，以确定抑制靶点。特别令人感兴趣的是烯丙醇（AA），它是蒜瓣研磨后积累的一种代谢产物。通过监测白色念珠菌细胞暴露于AA或市售大蒜提取物后细胞内反应的变化，研究了AA的假定靶点。使用了双光子激光扫描显微镜和其他技术。通过显微镜和流式细胞术观察到了典型的氧化应激变化——NADH氧化和谷胱甘肽消耗增加，以及活性氧增加。已知AA的靶点是醇脱氢酶Adh1和2（存在于细胞质中）以及Adh3（线粒体中的），尽管添加AA后NAD(P)H的显著降低表明存在另一种作用机制。
