Pitris Costas, Eracleous Peter
Department of Electrical and Computer Engineering, University of Cyprus, 75 Kallipoleos Street, Nicosia 1678, Cyprus.
Opt Lett. 2005 Oct 1;30(19):2590-2. doi: 10.1364/ol.30.002590.
The diagnostic utility of a conventional transillumination microscope, the most common imaging modality in clinical use today, is limited by the microscope's resolution. It is, however, possible to achieve lateral resolution well beyond the classical limit by using laterally structured illumination in a wide-field, nonconfocal microscope. In this method, the spatially modulated illumination (SMI) makes high-resolution information that is normally inaccessible visible in the observed image. Previously presented SMI microscopy systems operated in epifluorescence mode. We describe the design, construction, and testing of a novel transillumination SMI microscope. As transillumination is necessary for most medical applications, such as histopathologic evaluation of biopsy tissue and chromosomal analysis, such a system should have a significant diagnostic effect.
传统透照显微镜是当今临床使用中最常见的成像方式，其诊断效用受到显微镜分辨率的限制。然而，通过在宽视野、非共聚焦显微镜中使用横向结构照明，可以实现远超经典极限的横向分辨率。在这种方法中，空间调制照明（SMI）使通常无法获取的高分辨率信息在观察到的图像中可见。先前展示的SMI显微镜系统以落射荧光模式运行。我们描述了一种新型透照SMI显微镜的设计、构建和测试。由于透照对于大多数医学应用（如活检组织的组织病理学评估和染色体分析）是必要的，这样的系统应该具有显著的诊断效果。
