Zha Jianpeng, Fu Yan, Wu Yibing, Guo Chunhai, Zhang Diqun, Wang Yunzhi
New Medicine Development Laboratory, Hebei Medical University, Shijiazhuang.
Zhong Yao Cai. 2005 Jun;28(6):466-8.
The chemical components of the essential oil from Inula britannica L., which were collected from Yuncheng area of Shanxi province, were analyzed by GC-MS.
The essential oil was extracted from Inula britannica L. by steam distillation, the components were separated with the capillary chromatographic columns, the amount of the components from the essential oil was determined by normalization method. The components separated were identified by data search system. The chromatographic conditions were as follows: DB-5 (30m x 0.25mm, 0.25 microm) capillary column; High purity helium was used as carrier gas, and the flow rate was 1.0 ml/min; Column temperature: 70 degrees C keeping 2min, from 70 degrees C to 230 degrees C at rising rate 10 degrees C/min and keeping 10 min; Split ratio 15:1; Injector temperature 250 degrees C.
62 peaks were identified representing 68.4% of the total contents. Main component was 1-Benzoxepin-3-ol,2,3,4,5-tetrahydro-(4. 276% ).
The method is so reliable, stable, and good reproducible that can be applied to the analysis of the essential oil from Inula britannica L.
采用气相色谱-质谱联用（GC-MS）法分析采自山西省运城地区的大花旋覆花中挥发油的化学成分。
采用水蒸气蒸馏法从大花旋覆花中提取挥发油，用毛细管色谱柱进行分离，采用归一化法测定挥发油中各成分的含量。通过数据检索系统对分离得到的成分进行鉴定。色谱条件如下：DB-5（30m×0.25mm，0.25μm）毛细管柱；以高纯氦气为载气，流速为1.0ml/min；柱温：70℃保持2min，以10℃/min的速率从70℃升至230℃并保持10min；分流比15:1；进样口温度250℃。
共鉴定出62个峰，占总含量的68.4%。主要成分是1-苯并氧杂卓-3-醇，2,3,4,5-四氢-（4.276%）。
该方法可靠、稳定、重现性好，可用于大花旋覆花挥发油的分析。
