Matsuzaki Sachiko, Canis Michel, Vaurs-Barrière Catherine, Boespflug-Tanguy Odile, Dastugue Bernard, Mage Gérard
Department of Gynecology, Hŏtel-Dieu, Polyclinique, Centre Hospitalier Universitaire, Clermont-Ferrand, France.
Fertil Steril. 2005 Oct;84 Suppl 2:1180-90. doi: 10.1016/j.fertnstert.2005.04.041.
To investigate differentially expressed genes in epithelial and stromal cells of eutopic endometrium from patients with deep endometriosis and women with normal pelvic cavities using laser capture microdissection and complementary DNA microarrays.
Prospective study.
University hospital.
PATIENT(S): Patients with deep endometriosis and fertile women who underwent laparoscopic tubal ligation or reversal of tubal sterilization.
INTERVENTION(S): Endometrial tissue biopsies during the late proliferative phase and early, mid-, and late secretory phases.
MAIN OUTCOME MEASURE(S): Genes that were regulated with a change greater than threefold were selected as differentially expressed genes. Validation was performed with real-time reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULT(S): Microarray analysis identified up-regulation during the late secretory phase (patients with endometriosis vs. controls) of several genes in two important signaling pathways: RAS/RAF/MAPK and PI3K. This included the genes RON, SOS, 14-3-3 protein eta, and uPAR in epithelial cells and KSR and PI3K p85 regulatory subunit alpha in stromal cells; real-time RT-PCR analysis validated up-regulation of all six genes.
CONCLUSION(S): The RAS/RAF/MAPK and PI3K pathways may be involved in initial development of endometriosis.
运用激光捕获显微切割技术和互补DNA微阵列，研究深部子宫内膜异位症患者与盆腔正常女性在位内膜上皮细胞和基质细胞中的差异表达基因。
前瞻性研究。
大学医院。
深部子宫内膜异位症患者以及接受腹腔镜输卵管结扎术或输卵管绝育术复通术的育龄妇女。
在增殖期晚期以及分泌期早期、中期和晚期进行子宫内膜组织活检。
将变化大于三倍的调控基因选为差异表达基因。通过实时逆转录聚合酶链反应（RT-PCR）进行验证。
微阵列分析确定，在分泌期晚期（子宫内膜异位症患者与对照组相比），两条重要信号通路（RAS/RAF/MAPK和PI3K）中的多个基因上调。这包括上皮细胞中的RON、SOS、14-3-3蛋白eta和uPAR基因，以及基质细胞中的KSR和PI3K p85调节亚基α基因；实时RT-PCR分析验证了所有六个基因的上调。
RAS/RAF/MAPK和PI3K信号通路可能参与子宫内膜异位症的初始发展。
