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子宫内膜的激光捕获显微切割及cDNA阵列分析确定CCL16和CCL21为与子宫内膜异位症相关的上皮源性炎症介质。

Laser capture microdissection and cDNA array analysis of endometrium identify CCL16 and CCL21 as epithelial-derived inflammatory mediators associated with endometriosis.

作者信息

Chand Ashwini L, Murray Andrew S, Jones Rebecca L, Hannan Natalie J, Salamonsen Lois A, Rombauts Luk

机构信息

Prince Henry's Institute of Medical Research, Clayton, Victoria, Australia.

出版信息

Reprod Biol Endocrinol. 2007 May 17;5:18. doi: 10.1186/1477-7827-5-18.

DOI:10.1186/1477-7827-5-18
PMID:17506907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1884154/
Abstract

BACKGROUND

Understanding the pathophysiology of chemokine secretion in endometriosis may offer a novel area of therapeutic intervention. This study aimed to identify chemokines differentially expressed in epithelial glands in eutopic endometrium from normal women and those with endometriosis, and to establish the expression profiles of key chemokines in endometriotic lesions.

METHODS

Laser capture microdissection isolated epithelial glands from endometrial eutopic tissue from women with and without endometriosis in the mid-secretory phase of their menstrual cycles. Gene profiling of the excised glands used a human chemokine and receptor cDNA array. Selected chemokines were further examined using real-time PCR and immunohistochemistry.

RESULTS

22 chemokine/receptor genes were upregulated and two downregulated in pooled endometrial epithelium of women with endometriosis compared with controls. CCL16 and CCL21 mRNA was confirmed as elevated in some women with endometriosis compared to controls on individual samples. Immunoreactive CCL16 and CCL21 were predominantly confined to glands in eutopic and ectopic endometrium: leukocytes also stained. Immunoreactive CCL16 was overall higher in glands in ectopic vs. eutopic endometrium from the same woman (P < 0.05). Staining for CCL16 and CCL21 was highly correlated in individual tissues.

CONCLUSION

This study provides novel candidate molecules and suggests a potential local role for CCL16 and CCL21 as mediators contributing to the inflammatory events associated with endometriosis.

摘要

背景

了解子宫内膜异位症中趋化因子分泌的病理生理学可能为治疗干预提供一个新的领域。本研究旨在鉴定正常女性和子宫内膜异位症患者在位子宫内膜上皮腺体中差异表达的趋化因子，并建立子宫内膜异位症病灶中关键趋化因子的表达谱。

方法

在月经周期的分泌中期，采用激光捕获显微切割技术从有或无子宫内膜异位症的女性子宫内膜在位组织中分离上皮腺体。对切除的腺体进行基因谱分析，使用人趋化因子和受体cDNA阵列。对选定的趋化因子进一步采用实时PCR和免疫组织化学进行检测。

结果

与对照组相比，子宫内膜异位症患者合并的子宫内膜上皮中有22个趋化因子/受体基因上调，2个下调。在个体样本中，与对照组相比，一些子宫内膜异位症患者的CCL16和CCL21 mRNA被证实升高。免疫反应性CCL16和CCL21主要局限于在位和异位子宫内膜的腺体：白细胞也有染色。同一女性异位子宫内膜腺体中的免疫反应性CCL16总体上高于在位子宫内膜（P < 0.05）。CCL16和CCL21在个体组织中的染色高度相关。

结论

本研究提供了新的候选分子，并提示CCL16和CCL21作为促成与子宫内膜异位症相关炎症事件的介质可能发挥局部作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a255/1884154/a6b4d05b0b9c/1477-7827-5-18-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a255/1884154/33dbff5481ec/1477-7827-5-18-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a255/1884154/6ede00336744/1477-7827-5-18-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a255/1884154/6884af7b6881/1477-7827-5-18-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a255/1884154/a6b4d05b0b9c/1477-7827-5-18-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a255/1884154/33dbff5481ec/1477-7827-5-18-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a255/1884154/6ede00336744/1477-7827-5-18-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a255/1884154/6884af7b6881/1477-7827-5-18-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a255/1884154/a6b4d05b0b9c/1477-7827-5-18-4.jpg

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