使用组织蛋白质组学方法将胃蛋白酶原C鉴定为胃癌的潜在生物标志物
Characterization of pepsinogen C as a potential biomarker for gastric cancer using a histo-proteomic approach.
作者信息
Melle Christian, Ernst Günther, Schimmel Bettina, Bleul Annett, Kaufmann Roland, Hommann Merten, Richter Konrad K, Daffner Wolfgang, Settmacher Utz, Claussen Uwe, von Eggeling Ferdinand
机构信息
Core Unit Chip Application (CUCA), Institute of Human Genetics and Anthropology, Friedrich Schiller University, 07740 Jena, Germany.
出版信息
J Proteome Res. 2005 Sep-Oct;4(5):1799-804. doi: 10.1021/pr050123o.
We analyzed 74 cryostat sections of central gastric tumor, tumor margin, and normal gastric epithelium using ProteinChip Arrays and SELDI-TOF MS. One peak was significantly down-regulated in tumor tissue (P = 1.43 x 10(-6)) and identified as pepsinogen C using MS/MS analysis and immunodepletion. This signal was further characterized by immunohistochemistry. This work demonstrates that differentially expressed signals can be identified and assessed using a proteomic approach comprising tissue-microdissection, protein profiling, and immunohistochemistry.
我们使用蛋白质芯片阵列和表面增强激光解吸电离飞行时间质谱（SELDI-TOF MS）分析了74个胃中部肿瘤、肿瘤边缘及正常胃上皮的冷冻切片。一个峰在肿瘤组织中显著下调（P = 1.43 x 10(-6)），通过串联质谱（MS/MS）分析和免疫去除法鉴定为胃蛋白酶原C。该信号通过免疫组织化学进一步表征。这项工作表明，使用包括组织显微切割、蛋白质谱分析和免疫组织化学的蛋白质组学方法，可以识别和评估差异表达的信号。
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