胃癌的蛋白质组学分析及潜在生物标志物的免疫印迹验证。
Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers.
机构信息
Medical Centre for Molecular Biology, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana 1000, Slovenia.
出版信息
World J Gastroenterol. 2012 Mar 21;18(11):1216-28. doi: 10.3748/wjg.v18.i11.1216.
AIM
To search for and validate differentially expressed proteins in patients with gastric adenocarcinoma.
METHODS
We used two-dimensional gel electrophoresis and mass spectrometry to search for differentially expressed proteins in patients with gastric adenocarcinoma. A set of proteins was validated with immunoblotting.
RESULTS
We identified 30 different proteins involved in various biological processes: metabolism, development, death, response to stress, cell cycle, cell communication, transport, and cell motility. Eight proteins were chosen for further validation by immunoblotting. Our results show that gastrokine-1, 39S ribosomal protein L12 (mitochondrial precursor), plasma cell-induced resident endoplasmic reticulum protein, and glutathione S-transferase mu 3 were significantly underexpressed in gastric adenocarcinoma relative to adjacent non-tumor tissue samples. On the other hand, septin-2, ubiquitin-conjugating enzyme E2 N, and transaldolase were significantly overexpressed. Translationally controlled tumor protein was shown to be differentially expressed only in patients with cancer of the gastric cardia/esophageal border.
CONCLUSION
This work presents a set of possible diagnostic biomarkers, validated for the first time. It might contribute to the efforts of understanding gastric cancer carcinogenesis.
目的
寻找并验证胃腺癌患者中差异表达的蛋白质。
方法
我们使用二维凝胶电泳和质谱技术来寻找胃腺癌患者中差异表达的蛋白质。使用免疫印迹法验证了一组蛋白质。
结果
我们鉴定出了涉及各种生物学过程的 30 种不同的蛋白质：代谢、发育、死亡、应激反应、细胞周期、细胞通讯、运输和细胞运动。选择了 8 种蛋白质进行进一步的免疫印迹验证。我们的结果表明，胃泌素-1、39S 核糖体蛋白 L12（线粒体前体）、浆细胞诱导的驻留内质网蛋白和谷胱甘肽 S-转移酶 mu3 在胃腺癌中相对于相邻的非肿瘤组织样本明显低表达。另一方面， septin-2、泛素结合酶 E2N 和转醛醇酶明显过表达。翻译控制肿瘤蛋白仅在胃贲门/食管交界处的癌症患者中表现出差异表达。
结论
本研究首次验证了一组可能的诊断生物标志物，这可能有助于理解胃癌的发生机制。
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