Sprague Eugene A, Palmaz Julio C
Department of Radiology, The University of Texas Health Science Center at San Antonio, Texas 78229, USA.
J Endovasc Ther. 2005 Oct;12(5):594-604. doi: 10.1583/05-1555.1.
To establish a reproducible laboratory test to evaluate prospective vascular biomaterials with respect to their thromboinflammatory properties by examining fibrinogen, platelet, and monocyte binding. Endothelial migration onto these surfaces was used as an index of vascular healing.
To evaluate biomaterials for potential thrombogenicity and inflammation, binding assays of radiolabeled human fibrinogen, platelets, and monocytes were performed on standard pieces of vascular biomaterials, including metals and polymeric and ceramic-coated materials. Using an established in vitro endothelial cell migration model, the relative migration rate of cultured human aortic endothelial cells onto these vascular biomaterials was measured and compared. The fibrinogen, platelet, and monocyte binding results were combined along with the migration results to create an overall score of biocompatibility.
A significant direct relation of platelet and monocyte binding to the amount of adsorbed fibrinogen was observed. In contrast, migration rates of cultured human aortic endothelial cells onto the same biomaterial surfaces were found to be inversely related the amount of bound fibrinogen. Among the materials tested, stainless steel received the highest score of biocompatibility, while turbostratic carbon scored the lowest.
Fibrinogen, platelet, and monocyte binding levels, as well as endothelial migration rates onto vascular material surfaces, provide a basis for evaluating thrombogenicity, inflammatory potential, and endothelialization in the laboratory prior to in vivo testing.
通过检测纤维蛋白原、血小板和单核细胞的结合情况，建立一种可重复的实验室检测方法，以评估潜在的血管生物材料的血栓炎症特性。将内皮细胞在这些材料表面的迁移作为血管愈合的指标。
为评估生物材料的潜在血栓形成性和炎症反应，对包括金属、聚合物和陶瓷涂层材料在内的标准血管生物材料片进行放射性标记的人纤维蛋白原、血小板和单核细胞的结合试验。使用已建立的体外内皮细胞迁移模型，测量并比较培养的人主动脉内皮细胞在这些血管生物材料上的相对迁移率。将纤维蛋白原、血小板和单核细胞的结合结果与迁移结果相结合，得出生物相容性的总体评分。
观察到血小板和单核细胞的结合与吸附的纤维蛋白原量之间存在显著的直接关系。相反，发现培养的人主动脉内皮细胞在相同生物材料表面的迁移率与结合的纤维蛋白原量呈负相关。在所测试的材料中，不锈钢的生物相容性评分最高，而涡轮层状碳的评分最低。
纤维蛋白原、血小板和单核细胞的结合水平，以及内皮细胞在血管材料表面的迁移率，为在体内测试前在实验室评估血栓形成性、炎症潜力和内皮化提供了依据。
