Qin Weibing, Jiang Jianwei, Chen Qiaoer, Yang Ning, Wang Yifeng, Wei Xiangcai, Ou Ruqiang
Family Planning Research Institute of Guangdong, Guangzhou 510600, China.
Cell Mol Immunol. 2004 Apr;1(2):148-52.
Oligodeoxynucleotides (ODN) containing unmethylated CpG dinucleotides in contexts of unique sequence (CpG motifs) is active as adjuvant in induction of cellular and humoral immune responses in young mice. To date, there are only limited reports about effect of CpG ODN on immune responses against hepatitis B (HB) infection in aged mice. Our studies demonstrated there were significant increases in secreting of total anti-HB IgG, IgG1 and IgG2a, as well as of IL-12 and IFN-gamma, when CpG ODNs were injected together with hepatitis B antigen in aged mice. Moreover, CpG ODN could stimulate proliferation of spleen lymphocytes in a dose-dependent manner. Taken together, the results we obtained indicate that the adding of CpG ODN into the vaccine antigen might be useful in development of more effective vaccination for inducing anti-HB virus responses in the elderly.
在独特序列（CpG基序）背景下含有未甲基化CpG二核苷酸的寡脱氧核苷酸（ODN）作为佐剂在幼鼠诱导细胞免疫和体液免疫反应中具有活性。迄今为止，关于CpG ODN对老年小鼠抗乙型肝炎（HB）感染免疫反应影响的报道有限。我们的研究表明，当CpG ODN与乙型肝炎抗原一起注射到老年小鼠体内时，总抗HB IgG、IgG1和IgG2a以及IL-12和IFN-γ的分泌显著增加。此外，CpG ODN能以剂量依赖的方式刺激脾淋巴细胞增殖。综上所述，我们获得的结果表明，在疫苗抗原中添加CpG ODN可能有助于开发更有效的疫苗，以诱导老年人产生抗HB病毒反应。
