Wan Hui, Dupasquier Marcel
Department of Immunology, Erasmus MC, Rotterdam, Netherlands.
Cell Mol Immunol. 2005 Feb;2(1):28-35.
Dendritic cells (DC) are crucial cells of the immune system, and bridge the essential connection between innate and adaptive immunity. They reside in the periphery as sentinels where they take up antigens. Upon activation, they migrate to lymphoid organs and present there the processed antigens to T cells, thereby activating them and eliciting a potent immune response. Dendritic cells are bone marrow-derived cells, still big controversies exist about their in vivo development. In vitro, DC can be generated from multiple precursor cells, among them lymphoid and myeloid committed progenitors. Although it remains unknown how DC are generated in vivo, studying the functions of in vitro generated DC results in fundamental knowledge of the DC biology with promising applications for future medicine. Therefore, in this review, we present current protocols for the generation of DC from precursors in vitro. We will do this for the mouse system, where most research occurs and for the human system, where research concentrates on implementing DC biology in disease treatments.
树突状细胞（DC）是免疫系统的关键细胞，在先天性免疫和适应性免疫之间建立了重要联系。它们作为哨兵存在于外周组织，摄取抗原。激活后，它们迁移至淋巴器官，在那里将处理后的抗原呈递给T细胞，从而激活T细胞并引发强烈的免疫反应。树突状细胞来源于骨髓细胞，但其在体内的发育仍存在很大争议。在体外，DC可由多种前体细胞生成，包括淋巴系和髓系定向祖细胞。尽管目前尚不清楚DC在体内是如何生成的，但研究体外生成的DC的功能可以让我们深入了解DC生物学，为未来医学带来有前景的应用。因此，在本综述中，我们介绍了目前从小鼠和人源前体细胞体外生成DC的方案。小鼠系统是大多数研究的对象，而人类系统的研究则集中在将DC生物学应用于疾病治疗。
