Dracheva Stella, Davis Kenneth L, Chin Benjamin, Woo Derek A, Schmeidler James, Haroutunian Vahram
Department of Psychiatry, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA.
Neurobiol Dis. 2006 Mar;21(3):531-40. doi: 10.1016/j.nbd.2005.08.012. Epub 2005 Oct 5.
Microarray and other studies have reported oligodendrocyte and myelin-related (OMR) deficits in schizophrenia. Here, we employed a quantitative approach to determine the magnitude of OMR gene expression deficits and their brain-region specificity. In addition, we examined how expression levels among the studied OMR genes are interrelated. mRNA of MAG, CNP, SOX10, CLDN11, and PMP22, but not MBP and MOBP, was reduced in the hippocampus and anterior cingulate cortex but not in the putamen of patients with schizophrenia. Expression of the only protein examined (CNP) was decreased in the hippocampus but not in the putamen. Correlation and factor analyses revealed that mRNA levels for genes that did exhibit differential expression in schizophrenia (MAG, CNP, SOX10, CLDN11, and PMP2), as opposed to those that did not (MOBP and MBP), loaded on separate factors. Thus, OMR gene and protein expression deficits in schizophrenia are brain-region specific, and the affected components may share regulatory elements.
微阵列及其他研究报告了精神分裂症患者存在少突胶质细胞和髓鞘相关（OMR）缺陷。在此，我们采用定量方法来确定OMR基因表达缺陷的程度及其脑区特异性。此外，我们还研究了所研究的OMR基因之间的表达水平是如何相互关联的。精神分裂症患者海马体和前扣带回皮质中MAG、CNP、SOX10、CLDN11和PMP22的mRNA水平降低，但壳核中未降低，而MBP和MOBP的mRNA水平在壳核中未降低。所检测的唯一一种蛋白质（CNP）在海马体中的表达降低，但在壳核中未降低。相关性分析和因子分析表明，在精神分裂症中表现出差异表达的基因（MAG、CNP、SOX10、CLDN11和PMP2）的mRNA水平，与未表现出差异表达的基因（MOBP和MBP）的mRNA水平，分属于不同因子。因此，精神分裂症中的OMR基因和蛋白质表达缺陷具有脑区特异性，且受影响的成分可能共享调控元件。
