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迈向生命百科全书:DNA条形码技术简介

Towards writing the encyclopedia of life: an introduction to DNA barcoding.

作者信息

Savolainen Vincent, Cowan Robyn S, Vogler Alfried P, Roderick George K, Lane Richard

机构信息

Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK.

出版信息

Philos Trans R Soc Lond B Biol Sci. 2005 Oct 29;360(1462):1805-11. doi: 10.1098/rstb.2005.1730.

DOI:10.1098/rstb.2005.1730
PMID:16214739
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1609222/
Abstract

An international consortium of major natural history museums, herbaria and other organizations has launched an ambitious project, the 'Barcode of Life Initiative', to promote a process enabling the rapid and inexpensive identification of the estimated 10 million species on Earth. DNA barcoding is a diagnostic technique in which short DNA sequence(s) can be used for species identification. The first international scientific conference on Barcoding of Life was held at the Natural History Museum in London in February 2005, and here we review the scientific challenges discussed during this conference and in previous publications. Although still controversial, the scientific benefits of DNA barcoding include: (i) enabling species identification, including any life stage or fragment, (ii) facilitating species discoveries based on cluster analyses of gene sequences (e.g. cox1 = CO1, in animals), (iii) promoting development of handheld DNA sequencing technology that can be applied in the field for biodiversity inventories and (iv) providing insight into the diversity of life.

摘要

一个由大型自然历史博物馆、植物标本馆和其他组织组成的国际联盟发起了一项雄心勃勃的项目——“生命条形码计划”,以推动一个能够快速且低成本地识别地球上约1000万种物种的进程。DNA条形码是一种诊断技术,其中短DNA序列可用于物种识别。2005年2月,首届生命条形码国际科学会议在伦敦自然历史博物馆举行,在此我们回顾了本次会议以及以往出版物中讨论的科学挑战。尽管仍存在争议,但DNA条形码的科学益处包括:(i)能够识别物种,包括任何生命阶段或片段;(ii)基于基因序列的聚类分析(如动物中的cox1 = CO1)促进物种发现;(iii)推动可应用于野外生物多样性清查的手持式DNA测序技术的发展;(iv)深入了解生命的多样性。

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本文引用的文献

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Between two extremes: mitochondrial DNA is neither the panacea nor the nemesis of phylogenetic and taxonomic inference.介于两个极端之间:线粒体DNA既不是系统发育和分类学推断的万灵药,也不是其克星。
Syst Biol. 2005 Dec;54(6):952-61. doi: 10.1080/10635150500234674.
2
The promise of DNA barcoding for taxonomy.DNA条形码技术在分类学中的前景。
Syst Biol. 2005 Oct;54(5):852-9. doi: 10.1080/10635150500354886.
3
The perils of DNA barcoding and the need for integrative taxonomy.DNA条形码技术的风险及整合分类学的必要性。
Syst Biol. 2005 Oct;54(5):844-51. doi: 10.1080/10635150500354878.
4
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5
TaxI: a software tool for DNA barcoding using distance methods.TaxI:一种使用距离方法进行DNA条形码分析的软件工具。
Philos Trans R Soc Lond B Biol Sci. 2005 Oct 29;360(1462):1975-80. doi: 10.1098/rstb.2005.1729.
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A likelihood ratio test for species membership based on DNA sequence data.基于DNA序列数据的物种归属似然比检验。
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