A CARD-FISH protocol for the identification and enumeration of epiphytic bacteria on marine algae.

作者信息

Tujula Niina A, Holmström Carola, Mussmann Marc, Amann Rudolf, Kjelleberg Staffan, Crocetti Gregory R

机构信息

Centre for Marine Biofouling and Bio-Innovation, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.

出版信息

J Microbiol Methods. 2006 Jun;65(3):604-7. doi: 10.1016/j.mimet.2005.09.006. Epub 2005 Oct 10.

A CARD-FISH protocol was developed and applied to analyse surface-associated bacteria on the marine algae Ulva lactuca, Delisea pulchra, Corallina officinalis, Amphiroa anceps, Porphyra sp. and Sargassum linearifolium. The combination of Alexa(546)-labelled tyramide as the reporter molecule with SYBR Green II counterstain allowed for superior detection of the hybridised probe fluorescence against plant tissue from which pigment autofluorescence has been reduced.