从大型藻类表面选择性提取细菌DNA。
Selective extraction of bacterial DNA from the surfaces of macroalgae.
作者信息
Burke Catherine, Kjelleberg Staffan, Thomas Torsten
机构信息
School of Biotechnology and Biomolecular Sciences and Centre for Marine Bio-Innovation, The University of New South Wales, Sydney 2052, Australia.
出版信息
Appl Environ Microbiol. 2009 Jan;75(1):252-6. doi: 10.1128/AEM.01630-08. Epub 2008 Oct 31.
Abstract
A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra. The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, and was representative of the bacterial community on the algal surfaces. The DNA is suitable for a variety of subsequent applications, including the construction of large-insert clone libraries and metagenomic sequencing.
摘要
已经开发出一种新方法，用于从两种典型海洋底栖藻类——澳大利亚石莼（Ulva australis）和美丽鹿角菜（Delisea pulchra）的表面相关细菌群落中选择性提取DNA。提取的DNA未检测到宿主DNA污染，产量和质量都很高，并且能代表藻类表面的细菌群落。该DNA适用于多种后续应用，包括构建大插入片段克隆文库和宏基因组测序。
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