Hargrave Kimberly M, Azain Michael J, Miner Jess L
Department of Animal Science, C220 Animal Science, University of Nebraska, Lincoln, NE 68583, USA.
Biochim Biophys Acta. 2005 Oct 15;1737(1):52-60. doi: 10.1016/j.bbalip.2005.08.016. Epub 2005 Sep 13.
Conjugated linoleic acid (CLA) induces a body fat loss that is enhanced in mice fed coconut oil (CO), which lacks essential fatty acids (EFA). Our objective was to determine if CO enhancement of CLA-induced body fat loss is due to the lack of EFA. The CLA-EFA interaction was tested by feeding CO and fat free (FF) diets for varying times with and without replenishment of individual EFA. Mice fed CO during only the 2-week CLA-feeding period did not differ from control mice in their adipose EFA content but still tended (P=0.06) to be leaner than mice fed soy oil (SO). Mice raised on CO or FF diets and fed CLA were leaner than the SO+CLA-fed mice (P<0.01). Mice raised on CO and then replenished with linoleic, linolenic, or arachidonic acid were leaner when fed CLA than mice raised on SO (P<0.001). Body fat of CO+CLA-fed mice was not affected by EFA addition. In summary, CO-fed mice not lacking in tissue EFA responded more to CLA than SO-fed mice. Also, EFA addition to CO diets did not alter the enhanced response to CLA. Therefore, the increased response to CLA in mice raised on CO or FF diets appears to be independent of a dietary EFA deficiency.
共轭亚油酸（CLA）可促使机体脂肪减少，而在喂食缺乏必需脂肪酸（EFA）的椰子油（CO）的小鼠中，这种减脂效果会增强。我们的目标是确定CO增强CLA诱导的机体脂肪减少是否归因于缺乏EFA。通过在不同时间段喂食CO和无脂（FF）日粮，并补充或不补充单一EFA来测试CLA-EFA之间的相互作用。仅在为期2周的CLA喂食期内喂食CO的小鼠，其脂肪组织中的EFA含量与对照小鼠并无差异，但相较于喂食大豆油（SO）的小鼠，仍有体型更瘦的趋势（P = 0.06）。以CO或FF日粮饲养并喂食CLA的小鼠比喂食SO + CLA的小鼠更瘦（P < 0.01）。以CO饲养然后补充亚油酸、亚麻酸或花生四烯酸的小鼠，在喂食CLA时比以SO饲养的小鼠更瘦（P < 0.001）。添加EFA对喂食CO + CLA的小鼠的体脂没有影响。总之，组织中不缺乏EFA的喂食CO的小鼠对CLA的反应比喂食SO的小鼠更明显。此外，在CO日粮中添加EFA并不会改变对CLA增强的反应。因此，以CO或FF日粮饲养的小鼠对CLA反应的增强似乎与日粮中EFA缺乏无关。
