Wing Rod A, Ammiraju Jetty S S, Luo Meizhong, Kim Hyeran, Yu Yeisoo, Kudrna Dave, Goicoechea Jose L, Wang Wenming, Nelson Will, Rao Kiran, Brar Darshan, Mackill Dave J, Han Bin, Soderlund Cari, Stein Lincoln, SanMiguel Phillip, Jackson Scott
Arizona Genomics Institute, The University of Arizona, Tucson, AZ, USA.
Plant Mol Biol. 2005 Sep;59(1):53-62. doi: 10.1007/s11103-004-6237-x.
The wild species of the genus Oryza offer enormous potential to make a significant impact on agricultural productivity of the cultivated rice species Oryza sativa and Oryza glaberrima. To unlock the genetic potential of wild rice we have initiated a project entitled the 'Oryza Map Alignment Project' (OMAP) with the ultimate goal of constructing and aligning BAC/STC based physical maps of 11 wild and one cultivated rice species to the International Rice Genome Sequencing Project's finished reference genome--O. sativa ssp. japonica c. v. Nipponbare. The 11 wild rice species comprise nine different genome types and include six diploid genomes (AA, BB, CC, EE, FF and GG) and four tetrapliod genomes (BBCC, CCDD, HHKK and HHJJ) with broad geographical distribution and ecological adaptation. In this paper we describe our strategy to construct robust physical maps of all 12 rice species with an emphasis on the AA diploid O. nivara--thought to be the progenitor of modern cultivated rice.
稻属的野生稻种具有巨大潜力,可对栽培稻种亚洲栽培稻和非洲栽培稻的农业生产力产生重大影响。为了挖掘野生稻的遗传潜力,我们启动了一个名为“水稻图谱比对项目”(OMAP)的项目,其最终目标是构建11个野生稻种和1个栽培稻种基于细菌人工染色体/细菌单拷贝序列(BAC/STC)的物理图谱,并将其与国际水稻基因组测序计划完成的参考基因组——亚洲栽培稻粳稻亚种日本晴进行比对。这11个野生稻种包含9种不同的基因组类型,包括6个二倍体基因组(AA、BB、CC、EE、FF和GG)和4个四倍体基因组(BBCC、CCDD、HHKK和HHJJ),具有广泛的地理分布和生态适应性。在本文中,我们描述了构建所有12个水稻物种稳健物理图谱的策略,重点是被认为是现代栽培稻祖先的二倍体AA基因组野生稻——尼瓦拉野生稻。