Peng Youyuan, Ye Jiannong, Kong Jilie
Department of Chemistry, Fudan University, Shanghai 200433, China.
J Agric Food Chem. 2005 Oct 19;53(21):8141-7. doi: 10.1021/jf051360e.
A method based on capillary electrophoresis with electrochemical detection has been developed to analyze flavonoids and phenolic acids in Perilla frutescens L. for the first time. Catechin, ferulic acid, apigenin, luteolin, rosmarinic acid, and caffeic acid are major important active ingredients in the plant. Operated in a wall-jet configuration, a 300 microm diameter carbon-disk electrode was used as the working electrode, which exhibits a good response at 0.90 V (versus saturated calomel electrode) for the analytes. Under the optimum conditions, the analytes were baseline separated within 20 min in a 100 mmol/L borax buffer (pH 8.7). Notably, excellent linearity was obtained over 3 orders of magnitude with detection limits (S/N = 3) ranging from 2 x 10(-7) to 1 x 10(-6) g/mL for all analytes. This proposed method has been successfully applied to monitor the flavonoids and phenolic acids contents in the leaves and seeds of P. frutescens L. at different growth stages with relatively simple extraction procedures, and the assay results were satisfactory.
首次开发了一种基于毛细管电泳-电化学检测的方法来分析紫苏中的黄酮类化合物和酚酸。儿茶素、阿魏酸、芹菜素、木犀草素、迷迭香酸和咖啡酸是该植物中的主要重要活性成分。采用壁喷配置，使用直径为300微米的碳盘电极作为工作电极，该电极对分析物在0.90 V（相对于饱和甘汞电极）时表现出良好的响应。在最佳条件下，分析物在100 mmol/L硼砂缓冲液（pH 8.7）中20分钟内实现基线分离。值得注意的是，所有分析物在3个数量级范围内均获得了出色的线性，检测限（S/N = 3）为2×10(-7)至1×10(-6) g/mL。该方法已成功应用于监测不同生长阶段紫苏叶和种子中的黄酮类化合物和酚酸含量，提取过程相对简单，测定结果令人满意。
