Liedl Tim, Simmel Friedrich C
Department of Physics and Center for Nanoscience, Ludwig-Maximilians-Universität München, Geschwister-Scholl-Platz 1, 80539 Münich, Germany.
Nano Lett. 2005 Oct;5(10):1894-8. doi: 10.1021/nl051180j.
pH oscillations generated by a nonequilibrium chemical reaction are used to switch a pH-sensitive DNA structure between two distinct conformations. The utilization of a chemical oscillator represents a novel method for achieving autonomous motion in molecular devices. The oscillatory reaction is a variant of the Landolt reaction and produces pH variations in the range between pH 5 and 7. In this range, a cytosine-rich DNA strand can be switched between a random coil conformation and the folded i-motif structure. The conformational changes are monitored simultaneously with the pH value in fluorescence-resonance energy-transfer experiments.
由非平衡化学反应产生的pH振荡用于在两种不同构象之间切换pH敏感的DNA结构。利用化学振荡器代表了一种在分子装置中实现自主运动的新方法。振荡反应是Landolt反应的一种变体，产生pH值在5至7之间的变化。在此范围内，富含胞嘧啶的DNA链可以在无规卷曲构象和折叠的i-基序结构之间切换。在荧光共振能量转移实验中，构象变化与pH值同时进行监测。
