Bergmans L, Moisiadis P, Van Meerbeek B, Quirynen M, Lambrechts P
Leuven BIOMAT Research Cluster, School of Dentistry, Oral Pathology and Maxillo-Facial Surgery, Catholic University of Leuven, Leuven, Belgium.
Int Endod J. 2005 Nov;38(11):775-88. doi: 10.1111/j.1365-2591.2005.00999.x.
Throughout the years, various methods have been adopted to investigate bacteria involved in root canal infection and apical periodontitis. This paper reviews the most commonly used microscopic techniques and discusses their possibilities, limitations and sample preparation. In particular, a recently developed variant of scanning electron microscope (SEM), referred to as environmental SEM (ESEM), is highlighted due to its potential impact across the diverse field of biomaterials research. The performance of this ESEM technique for bacterial observation of endodontic pathogens was illustrated by a practical approach. The paper concludes with a discussion on the possible use of ESEM for testing endodontic treatment modalities under environmental conditions in situ.
多年来，人们采用了各种方法来研究参与根管感染和根尖周炎的细菌。本文回顾了最常用的显微镜技术，并讨论了它们的可能性、局限性和样品制备。特别是，一种最近开发的扫描电子显微镜（SEM）变体，即环境扫描电子显微镜（ESEM），因其在生物材料研究的不同领域的潜在影响而受到关注。通过一种实际方法展示了这种ESEM技术用于牙髓病原体细菌观察的性能。本文最后讨论了ESEM在原位环境条件下测试牙髓治疗方式的可能用途。
