Mitra Sanchita, Banka Nagapriya, Basu Soumyava, Rao Tirupathi
Consultant Microbiologist, Jhaveri Microbiology Centre, LV Prasad Eye Institute, Kallam Anji Reddy Campus, Hyderabad, 500034, India.
Senior Technician, Scanning Electron Microscopy, LV Prasad Eye Institute, Kallam Anji Reddy Campus, Hyderabad, 500034, India.
Future Microbiol. 2025 Feb;20(2):117-135. doi: 10.1080/17460913.2024.2417617. Epub 2024 Nov 6.
To investigate antagonistic interactions among pathogens, in donor corneas infected with monomicrobial or polymicrobial combinations of antibiotic susceptible and resistant clinical isolates of (MSSA, MRSA) and (S-PA, MDR-PA). Scanning electron microscopy and antimicrobial susceptibility testing (AST, broth microdilution for minimum inhibitory and bactericidal concentrations [MIC/MBC]) pre-and post-polymicrobial interactions, in infected donor corneas. MSSA lost viability with S-PA/MDR-PA, while MRSA formed larger cells, biofilm and lower MIC (teicoplanin) with S-PA, but lost viability with MDR-PA. S-PA had lower MIC (ceftazidime, meropenem, chloramphenicol) with MSSA, and lower MBC (cefoperazone, ciprofloxacin) and fewer cells with MRSA. MDR-PA had abundant cells and no change in AST with MSSA or MRSA. Significant antagonistic interactions occur in ocular polymicrobial infections, affecting antibiotic susceptible isolates more than resistant ones.
为了研究病原体之间的拮抗相互作用，在供体角膜中接种了耐抗生素和抗生素敏感的临床分离株（甲氧西林敏感金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌）和（敏感铜绿假单胞菌、多重耐药铜绿假单胞菌）的单一微生物或多微生物组合。在感染的供体角膜中，通过扫描电子显微镜和抗菌药敏试验（AST，肉汤微量稀释法测定最低抑菌浓度和杀菌浓度[MIC/MBC]）检测多微生物相互作用前后的情况。甲氧西林敏感金黄色葡萄球菌与敏感铜绿假单胞菌/多重耐药铜绿假单胞菌共同培养时活力丧失，而耐甲氧西林金黄色葡萄球菌与敏感铜绿假单胞菌共同培养时形成更大的细胞、生物膜且最低抑菌浓度（替考拉宁）降低，但与多重耐药铜绿假单胞菌共同培养时活力丧失。敏感铜绿假单胞菌与甲氧西林敏感金黄色葡萄球菌共同培养时最低抑菌浓度（头孢他啶、美罗培南、氯霉素）降低，与耐甲氧西林金黄色葡萄球菌共同培养时最低杀菌浓度（头孢哌酮、环丙沙星）降低且细胞数量减少。多重耐药铜绿假单胞菌与甲氧西林敏感金黄色葡萄球菌或耐甲氧西林金黄色葡萄球菌共同培养时细胞数量丰富且抗菌药敏试验无变化。眼部多微生物感染中发生显著的拮抗相互作用，对抗生素敏感分离株的影响大于耐药分离株。
