Ishikawa Satoru, Ae Noriharu, Yano Masahiro
Department of Environmental Chemistry, Heavy Metal Group, Soil Biochemistry Unit, National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, Japan.
New Phytol. 2005 Nov;168(2):345-50. doi: 10.1111/j.1469-8137.2005.01516.x.
A novel mapping population consisting of 39 chromosome segment substitution lines (CSSLs) was used to locate the putative quantitative trait loci (QTLs) for cadmium (Cd) concentration in brown rice (Oryza sativa). The mapping population carried a single chromosome segment of 'Kasalath' (indica) in each line overlapping with neighbouring segments in a 'Koshihikari' (japonica) genetic background. The parents and CSSLs were grown in pots filled with Cd-polluted soil until grain filling. The brown rice of three of the 39 CSSLs had significantly lower Cd concentrations than that of Koshihikari, and the brown rice of a further three had significantly higher concentrations. On the basis of graphical genotypes of CSSLs, putative QTLs controlling the Cd concentration in brown rice were detected on chromosomes 3, 6 and 8. Each of the CSSLs was nearly isogenic to Koshihikari, which is the most popular rice cultivar in Japan: they carried > 90% of the Koshihikari genetic background. Therefore, the development of a new Koshihikari with less Cd concentration in brown rice would be feasible in the near future.
利用一个由39个染色体片段代换系（CSSLs）组成的新型作图群体，定位了糙米（水稻）中镉（Cd）浓度的假定数量性状位点（QTLs）。该作图群体中每个株系携带一个来自“Kasalath”（籼稻）的单一染色体片段，在“越光”（粳稻）遗传背景下与相邻片段重叠。亲本和CSSLs种植在装有镉污染土壤的花盆中，直至灌浆期。39个CSSLs中有3个株系的糙米镉浓度显著低于越光，另有3个株系的糙米镉浓度显著高于越光。基于CSSLs的图形基因型，在第3、6和8号染色体上检测到控制糙米镉浓度的假定QTLs。每个CSSLs与日本最流行的水稻品种越光几乎近等基因：它们携带了超过90%的越光遗传背景。因此，在不久的将来培育出糙米镉浓度较低的新型越光品种是可行的。
