Nagata Kazufumi, Nonoue Yasunori, Matsubara Kazuki, Mizobuchi Ritsuko, Ono Nozomi, Shibaya Taeko, Ebana Kaworu, Ogiso-Tanaka Eri, Tanabata Takanari, Sugimoto Kazuhiko, Taguchi-Shiobara Fumio, Yonemaru Jun-Ichi, Uga Yusaku, Fukuda Atsunori, Ueda Tadamasa, Yamamoto Shin-Ichi, Yamanouchi Utako, Takai Toshiyuki, Ikka Takashi, Kondo Katsuhiko, Hoshino Tomoki, Yamamoto Eiji, Adachi Shunsuke, Sun Jian, Kuya Noriyuki, Kitomi Yuka, Iijima Ken, Nagasaki Hideki, Shomura Ayahiko, Mizubayashi Tatsumi, Kitazawa Noriyuki, Hori Kiyosumi, Ando Tsuyu, Yamamoto Toshio, Fukuoka Shuichi, Yano Masahiro
National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8518, Japan.
Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Japan.
Breed Sci. 2023 Jun;73(3):332-342. doi: 10.1270/jsbbs.23006. Epub 2023 Jun 27.
Many agronomic traits that are important in rice breeding are controlled by multiple genes. The extensive time and effort devoted so far to identifying and selecting such genes are still not enough to target multiple agronomic traits in practical breeding in Japan because of a lack of suitable plant materials in which to efficiently detect and validate beneficial alleles from diverse genetic resources. To facilitate the comprehensive analysis of genetic variation in agronomic traits among Asian cultivated rice, we developed 12 sets of chromosome segment substitution lines (CSSLs) with the background, 11 of them in the same genetic background, using donors representing the genetic diversity of Asian cultivated rice. Using these materials, we overviewed the chromosomal locations of 1079 putative QTLs for seven agronomic traits and their allelic distribution in Asian cultivated rice through multiple linear regression analysis. The CSSLs will allow the effects of putative QTLs in the highly homogeneous background to be validated.
许多在水稻育种中重要的农艺性状是由多个基因控制的。由于缺乏合适的植物材料来有效地检测和验证来自不同遗传资源的有益等位基因，到目前为止，在日本实际育种中用于鉴定和选择此类基因所投入的大量时间和精力仍不足以针对多个农艺性状。为了促进对亚洲栽培稻农艺性状遗传变异的全面分析，我们利用代表亚洲栽培稻遗传多样性的供体，开发了12套染色体片段代换系（CSSLs），其中11套具有相同的遗传背景。利用这些材料，我们通过多元线性回归分析概述了亚洲栽培稻中7个农艺性状的1079个假定QTL的染色体位置及其等位基因分布。这些CSSLs将使在高度同质背景下假定QTL的效应得以验证。
