Gao Wenzhong, Xing Bengang, Tsien Roger Y, Rao Jianghong
Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, California 90095-1770, USA.
J Am Chem Soc. 2003 Sep 17;125(37):11146-7. doi: 10.1021/ja036126o.
A new class of small nonfluorescent fluorogenic substrates becomes brightly fluorescent after beta-lactamase hydrolysis with up to 153-fold enhancement in the fluorescence intensity. Less than 500 fM of beta-lactamase in cell lysates can be readily detected, and beta-lactamase expression in living cells can be imaged with a red fluorescence derivative. These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of beta-lactamase as a biosensor.
一类新型的非荧光小分子荧光底物在β-内酰胺酶水解后会发出明亮的荧光，荧光强度增强高达153倍。细胞裂解物中低至500 fM的β-内酰胺酶都能被轻易检测到，并且活细胞中β-内酰胺酶的表达可用一种红色荧光衍生物进行成像。这些新型荧光底物应可用于临床诊断，并促进β-内酰胺酶作为生物传感器的应用。
