用于成像β-内酰胺酶基因表达的新型荧光底物。
Novel fluorogenic substrates for imaging beta-lactamase gene expression.
作者信息
Gao Wenzhong, Xing Bengang, Tsien Roger Y, Rao Jianghong
机构信息
Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, California 90095-1770, USA.
出版信息
J Am Chem Soc. 2003 Sep 17;125(37):11146-7. doi: 10.1021/ja036126o.
A new class of small nonfluorescent fluorogenic substrates becomes brightly fluorescent after beta-lactamase hydrolysis with up to 153-fold enhancement in the fluorescence intensity. Less than 500 fM of beta-lactamase in cell lysates can be readily detected, and beta-lactamase expression in living cells can be imaged with a red fluorescence derivative. These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of beta-lactamase as a biosensor.
一类新型的非荧光小分子荧光底物在β-内酰胺酶水解后会发出明亮的荧光，荧光强度增强高达153倍。细胞裂解物中低至500 fM的β-内酰胺酶都能被轻易检测到，并且活细胞中β-内酰胺酶的表达可用一种红色荧光衍生物进行成像。这些新型荧光底物应可用于临床诊断，并促进β-内酰胺酶作为生物传感器的应用。
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