利用头孢菌素类荧光探针特异性检测 IMP-1β-内酰胺酶活性。
Specific detection of IMP-1 β-lactamase activity using a cephalosporin-based fluorogenic probe.
机构信息
State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
Shanghai Soong Ching Ling School, Shanghai 201703, China.
出版信息
Chem Commun (Camb). 2021 Dec 14;57(99):13586-13589. doi: 10.1039/d1cc05955f.
A fluorogenic probe for the specific detection of IMP-1 β-lactamase activity has been developed. This imaging reagent features a unique -acetylamino cephalosporin as an enzymatic recognition moiety, exhibiting excellent selectivity to IMP-1 β-lactamase over other β-lactamases, including serine- and metallo-β-lactamases. The selective activation of the probe by IMP-1 β-lactamase leads to over 30-fold enhancement in the fluorescence intensity, which allows enzyme activity to be reported with high sensitivity.
现已开发出一种用于特异性检测 IMP-1 β-内酰胺酶活性的荧光探针。这种成像试剂的特点是采用独特的 -乙酰氨基头孢菌素作为酶识别部分，对 IMP-1 β-内酰胺酶具有优异的选择性，超过其他 β-内酰胺酶，包括丝氨酸和金属 β-内酰胺酶。IMP-1 β-内酰胺酶对探针的选择性激活导致荧光强度增强超过 30 倍，从而可以高灵敏度报告酶活性。
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