Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Chem Commun (Camb). 2010 Oct 21;46(39):7403-5. doi: 10.1039/c0cc02432e. Epub 2010 Sep 8.
The targeted protein of interest is fused with genetically modified β-lactamase enzyme, which reacts with the probe in physiological conditions to break the aggregated interaction between the fluorophore and quencher. This alliance-separation technique is new for protein labeling and is probed in vitro and in live cell imaging studies.
目的蛋白与经过基因改造的β-内酰胺酶融合，在生理条件下与探针反应，打破荧光团和猝灭剂之间的聚集相互作用。这种联盟分离技术是一种新的蛋白质标记方法，已经在体外和活细胞成像研究中进行了探索。
