Ogunseitan O A, Sayler G S, Miller R V
Department of Microbiology, University of Tennessee, Knoxville 37996.
Appl Environ Microbiol. 1992 Jun;58(6):2046-52. doi: 10.1128/aem.58.6.2046-2052.1992.
Radiolabeled bacteriophage DNA probes have been used in this study to determine the distribution of Pseudomonas aeruginosa-infecting bacteriophages in natural samples of lake water, sediment, soil, and sewage. The sensitivity of detection of bacteriophage with the DNA probes was between 10(3) and 10(4) PFU and 10(6) to 10(7) CFU of lysogenized bacteria detectable with a homologous phage DNA probe. Analyses of environmental samples suggest that up to 40% of P. aeruginosa in natural ecosystems contain DNA sequences homologous to phage genomes. By using different bacteriophage DNA probes, the diversity of the bacteriophage population in sewage was estimated to be higher than that in other natural samples. The indication that transducing phages and prophages are widely distributed in the Pseudomonas populations investigated has considerable implications for the frequency of natural gene transfer by transduction and of lysogenic conversion of host bacteria in natural ecosystems.
在本研究中，放射性标记的噬菌体DNA探针已被用于确定铜绿假单胞菌感染噬菌体在湖水、沉积物、土壤和污水等天然样品中的分布。用DNA探针检测噬菌体的灵敏度为10³至10⁴噬菌斑形成单位（PFU），而用同源噬菌体DNA探针可检测到10⁶至10⁷溶源化细菌的菌落形成单位（CFU）。对环境样品的分析表明，自然生态系统中高达40%的铜绿假单胞菌含有与噬菌体基因组同源的DNA序列。通过使用不同的噬菌体DNA探针，估计污水中噬菌体群体的多样性高于其他天然样品。转导噬菌体和原噬菌体在所研究的假单胞菌群体中广泛分布，这一迹象对于自然生态系统中通过转导进行自然基因转移的频率以及宿主细菌的溶源转化具有重要意义。
