Kilbane J J, Miller R V
Department of Biochemistry and Biophysics, Loyola University of Chicago, Stritch School of Medicine, Maywood, Illinois 60153.
Virology. 1988 May;164(1):193-200. doi: 10.1016/0042-6822(88)90636-8.
We have characterized a new phage, B86, of Pseudomonas aeruginosa isolated from nature. It is a temperate, uv-inducible, generalized transducing phage. To determine the relatedness of this phage to other characterized P. aeruginosa phages, DNA homology studies were carried out. P. aeruginosa phages have previously been grouped by immunological cross-reactivity. Our studies confirm this classification by demonstrating that phages of different class share little or no DNA homology. Based on homology studies as well as cross-immunity to superinfection, B86 is related to other class B phages and is most homologous with phage B39. The virion morphology of these two phages is quite different, however, as are the restriction enzyme digestion patterns of their genomes with several restriction enzymes. Wild-type B86 is subject to the host-controlled restriction-modification systems of P. aeruginosa PAO and PAT. Virulent mutants of this phage are not restricted by these same restriction-modification systems.
我们已对从自然界分离出的一株铜绿假单胞菌新噬菌体B86进行了特性鉴定。它是一种温和的、紫外线可诱导的、普遍性转导噬菌体。为了确定该噬菌体与其他已鉴定的铜绿假单胞菌噬菌体的亲缘关系，我们进行了DNA同源性研究。铜绿假单胞菌噬菌体先前已通过免疫交叉反应进行分组。我们的研究通过证明不同类别的噬菌体几乎没有或没有DNA同源性，证实了这一分类。基于同源性研究以及对超感染的交叉免疫，B86与其他B类噬菌体相关，并且与噬菌体B39的同源性最高。然而，这两种噬菌体的病毒体形态有很大不同，它们基因组的几种限制酶消化模式也不同。野生型B86受铜绿假单胞菌PAO和PAT的宿主控制的限制修饰系统的影响。该噬菌体的烈性突变体不受这些相同的限制修饰系统的限制。
