Akhverdian V Z, Khrenova E A, Bogush V G, Gerasimova T V, Kirsanov N B
Genetika. 1984 Oct;20(10):1612-9.
Five phages (PH2, PH51, PH59, PH93 and PH132) which have some characteristics common with D3112, the transposable phage of Pseudomonas aeruginosa, were isolated from clinical P. aeruginosa isolates. The phages were distributed into 4 different immunity groups. The basic criteria used for selection of transposable phages have been: 1) Morphology of a phage particle, host range, similar inactivation with antiserum; 2) Similar sizes of phage genomes; 3) The presence of a variable non-phage nucleotide sequences covalently linked to phage genome DNA, which could be identified using restriction endonucleases or by heteroduplex analyses. The DNAs of the new phages are resistant to treatment with BamH1 endonuclease, like the DNAs of phages D3112, B39 and B3 described earlier. The restriction maps of the phage genomes are constructed.
从临床分离的铜绿假单胞菌中分离出5种噬菌体（PH2、PH51、PH59、PH93和PH132），它们具有与铜绿假单胞菌转座噬菌体D3112的一些共同特征。这些噬菌体被分为4个不同的免疫组。选择转座噬菌体所使用的基本标准如下：1）噬菌体颗粒的形态、宿主范围、与抗血清的相似失活情况；2）噬菌体基因组的相似大小；3）存在与噬菌体基因组DNA共价连接的可变非噬菌体核苷酸序列，可使用限制性内切酶或通过异源双链分析来鉴定。与之前描述的噬菌体D3112、B39和B3的DNA一样，新噬菌体的DNA对BamH1内切酶处理具有抗性。构建了噬菌体基因组的限制性图谱。
